Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

An Optimized Ustilago Chassis for Itaconic Acid Production at Theoretical Maximal Yield

Version 1 : Received: 22 November 2020 / Approved: 23 November 2020 / Online: 23 November 2020 (13:47:27 CET)

A peer-reviewed article of this Preprint also exists.

Becker, J.; Hosseinpour Tehrani, H.; Ernst, P.; Blank, L.M.; Wierckx, N. An Optimized Ustilago Maydis for Itaconic Acid Production at Maximal Theoretical Yield. J. Fungi 2021, 7, 20. Becker, J.; Hosseinpour Tehrani, H.; Ernst, P.; Blank, L.M.; Wierckx, N. An Optimized Ustilago Maydis for Itaconic Acid Production at Maximal Theoretical Yield. J. Fungi 2021, 7, 20.

Journal reference: J. Fungi 2020, 7, 20
DOI: 10.3390/jof7010020

Abstract

Ustilago maydis, member of the Ustilaginaceae family, is a promising host for the production of several metabolites including itaconic acid. This dicarboxylate has great potential as a bio-based building block in the polymer industry, and is of special interest for pharmaceutical applications. Several itaconate overproducing Ustilago strains have been generated by metabolic and morphology engineering. This yielded stabilized unicellular morphology through fuz7 deletion, reduction of by-product formation through deletion of genes responsible for itaconate oxidation and (glyco)lipid production, and the overexpression of the regulator of the itaconate cluster ria1 and the mitochondrial tricarboxylate transporter encoded by mttA from Aspergillus terreus. In this study, itaconate production was further optimized by consolidating these different optimizations into one strain. The combined modifications resulted in itaconic acid production at theoretical maximal yield, which was achieved under biotechnologically relevant fed-batch fermentations with continuous feed.

Subject Areas

Itaconic acid; U. maydis; Metabolic engineering; Fungi; Yeast

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