Working Paper Article Version 1 This version is not peer-reviewed

Application of Autologous Peripheral Blood Mononuclear Cells into the Area of Spinal Cord Injury in a Subacute Period: A Pilot Study in Pigs

Iliya Shulman
,
Sergei Ogurcov
,
Alexander Kostennikov
,
Alexander Rogozin
,
Ekaterina Garanina
,
Galina Masgutova
,
Mikhail Sergeev
,
Albert Rizvanov
,
Yana Mukhamedshina
*
Version 1 : Received: 17 November 2020 / Approved: 18 November 2020 / Online: 18 November 2020 (13:00:26 CET)

How to cite: Shulman, I.; Ogurcov, S.; Kostennikov, A.; Rogozin, A.; Garanina, E.; Masgutova, G.; Sergeev, M.; Rizvanov, A.; Mukhamedshina, Y. Application of Autologous Peripheral Blood Mononuclear Cells into the Area of Spinal Cord Injury in a Subacute Period: A Pilot Study in Pigs. Preprints 2020, 2020110478 Shulman, I.; Ogurcov, S.; Kostennikov, A.; Rogozin, A.; Garanina, E.; Masgutova, G.; Sergeev, M.; Rizvanov, A.; Mukhamedshina, Y. Application of Autologous Peripheral Blood Mononuclear Cells into the Area of Spinal Cord Injury in a Subacute Period: A Pilot Study in Pigs. Preprints 2020, 2020110478

Abstract

Peripheral blood is an available source of adult stem cells for both fundamental research and clinical use. Peripheral blood mononuclear cells (PBMCs) contain a number of different multipotent populations of progenitor cells and possess a potential to differentiate into various types of cells under appropriate conditions. We have evaluated a regenerative potential of PBMCs embedded into fibrin matrix (FM) in a model of pig spinal cord injury. The distribution of transplanted PBMCs in the injured spinal cord was previously evaluated, which demonstrated the ability of these cells to penetrate into deep layers of the spinal cord and to concentrate mainly in the grey matter. The pilot study conducted demonstrated that the tissue structure could be improved and the conduction along posterior columns of the spinal cord could be partially recovered in animals in the setting of FM+РВМCs application. The multiplex analysis of blood serum and cerebrospinal fluid (CSF) showed the cytokine imbalance to occur without significantly shifting towards pro-inflammatory or anti-inflammatory cytokine cascades in the setting therapy provided.

Keywords

peripheral blood mononuclear cells; spinal cord injury; pigs; fibrin matrix

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.