Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Targeted-Protein Silencing Tools: Overview and Future Perspectives

Version 1 : Received: 30 October 2020 / Approved: 2 November 2020 / Online: 2 November 2020 (15:59:46 CET)

How to cite: Prozzillo, Y.; Fattorini, G.; Santopietro, M.V.; Suglia, L.; Ruggiero, A.; Ferreri, D.; Messina, G. Targeted-Protein Silencing Tools: Overview and Future Perspectives. Preprints 2020, 2020110061 (doi: 10.20944/preprints202011.0061.v1). Prozzillo, Y.; Fattorini, G.; Santopietro, M.V.; Suglia, L.; Ruggiero, A.; Ferreri, D.; Messina, G. Targeted-Protein Silencing Tools: Overview and Future Perspectives. Preprints 2020, 2020110061 (doi: 10.20944/preprints202011.0061.v1).

Abstract

Targeted Protein Silencing (TPS) is an elegant approach to investigate protein function and its role in the cellular landscape, overcoming limitations of genetic perturbation strategies. In contrast to CRISPR/Cas9 and RNA interference, these systems act in a reversible manner and reduce off-target effects. Several TPS have been developed and wisely improved, including compartment delocalization tools and protein degradation systems. In this review, we focus on Anchor-Away, deGradFP, auxin inducible degron (AID) and dTAG technologies, and discuss their recent applications and advances. Finally, we propose Nano-Grad, a novel nanobody-based protein degradation tool to specifically proteolyze endogenous tag-free target protein.

Subject Areas

Targeted Protein Silencing (TPS); Targeted Protein Degradation (TPD); dTAG; FKBP12; von Hippel-Lindau (VHL); degron; deGradFP; Anchor-Away; Nanobody; Nano-Grad

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