Submitted:
30 October 2020
Posted:
02 November 2020
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Abstract
Targeted Protein Silencing (TPS) is an elegant approach to investigate protein function and its role in the cellular landscape, overcoming limitations of genetic perturbation strategies. In contrast to CRISPR/Cas9 and RNA interference, these systems act in a reversible manner and reduce off-target effects. Several TPS have been developed and wisely improved, including compartment delocalization tools and protein degradation systems. In this review, we focus on Anchor-Away, deGradFP, auxin inducible degron (AID) and dTAG technologies, and discuss their recent applications and advances. Finally, we propose Nano-Grad, a novel nanobody-based protein degradation tool to specifically proteolyze endogenous tag-free target protein.
Keywords:
Targeted Protein Silencing (TPS)
; Targeted Protein Degradation (TPD)
; dTAG
; FKBP12
; von Hippel-Lindau (VHL)
; degron
; deGradFP
; Anchor-Away
; Nanobody
; Nano-Grad
Copyright: This open access article is published under a Creative Commons CC BY 4.0 license, which permit the free download, distribution, and reuse, provided that the author and preprint are cited in any reuse.
