Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Comparative Genomics Reveals Novel Target Genes Towards Specific Control of Plant-Parasitic Nematodes

Version 1 : Received: 20 October 2020 / Approved: 22 October 2020 / Online: 22 October 2020 (09:01:30 CEST)

A peer-reviewed article of this Preprint also exists.

Grynberg, P.; Coiti Togawa, R.; Dias de Freitas, L.; Antonino, J.D.; Rancurel, C.; Mota do Carmo Costa, M.; Grossi-de-Sa, M.F.; Miller, R.N.G.; Brasileiro, A.C.M.; Messenberg Guimaraes, P.; Danchin, E.G.J. Comparative Genomics Reveals Novel Target Genes towards Specific Control of Plant-Parasitic Nematodes. Genes 2020, 11, 1347. Grynberg, P.; Coiti Togawa, R.; Dias de Freitas, L.; Antonino, J.D.; Rancurel, C.; Mota do Carmo Costa, M.; Grossi-de-Sa, M.F.; Miller, R.N.G.; Brasileiro, A.C.M.; Messenberg Guimaraes, P.; Danchin, E.G.J. Comparative Genomics Reveals Novel Target Genes towards Specific Control of Plant-Parasitic Nematodes. Genes 2020, 11, 1347.

Journal reference: Genes 2020, 11, 1347
DOI: 10.3390/genes11111347

Abstract

Plant-parasitic nematodes cause expressive annual yield losses to worldwide agricultural production. Most cultivated plants have no known resistance against nematodes and the few bearing a resistance gene can be overcome by certain species. The chemical methods that have been deployed to control nematodes were largely banned from use due to their poor specificity and high toxicity. Hence, there is an urgent need for the development of cleaner and more specific control methods. Recent advances in nematode genomics, including in phytoparasitic species, provide an unprecedented opportunity to identify genes and functions specific to these pests. Using phylogenomics, we compared 61 nematode genomes, including 16 for plant-parasitic species and identified more than 24,000 protein families specific to these parasites. In the genome of Meloidogyne incognita, one of the most devastating plant parasites, we found ca. 10,000 proteins with orthologs restricted only to phytoparasitic species and no further homology in protein databases. Among these phytoparasites-specific proteins, ca. 1,000 shared the same properties as known secreted effectors involved in essential parasitic functions. Of those, 68 were novel and showed strong expression during the endophytic phase of the nematode life cycle, based on both RNA-seq and RT-qPCR analyses. Besides effector candidates, transcription-related and neuro-perception functions were enriched in phytoparasites-specific proteins, revealing interesting targets for nematode control methods. This phylogenomics analysis, constitutes an unprecedented resource for the further understanding of the genetic basis of nematode adaptation to phytoparasitism and for the development of more efficient control methods.

Subject Areas

comparative genomics; plant-parasitic nematodes; phylogenomics; parasite-specific genes; pest control; de novo gene birth; horizontal gene transfers

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.