Working Paper Article Version 2 This version is not peer-reviewed

Silica Nanoparticle Effect on Population Parameters and Gene Expression of an Internal Feeder, American Serpentine Leafminer

Version 1 : Received: 17 October 2020 / Approved: 19 October 2020 / Online: 19 October 2020 (10:54:13 CEST)
Version 2 : Received: 5 December 2020 / Approved: 7 December 2020 / Online: 7 December 2020 (11:26:40 CET)

How to cite: Thabet, A.F.; Galal, O.A.; Tuda, M.; El–Samahy, M.F.; Fujita, R.; Hino, M. Silica Nanoparticle Effect on Population Parameters and Gene Expression of an Internal Feeder, American Serpentine Leafminer. Preprints 2020, 2020100369 Thabet, A.F.; Galal, O.A.; Tuda, M.; El–Samahy, M.F.; Fujita, R.; Hino, M. Silica Nanoparticle Effect on Population Parameters and Gene Expression of an Internal Feeder, American Serpentine Leafminer. Preprints 2020, 2020100369

Abstract

Insects quickly develop resistance to conventional chemical insecticides. The use of silica nanoparticles (SiO2NPs) is a promising new approach for managing insect pests. The insects that have been studied are, in most cases, external feeders and internal leaf feeders have not been well studied. Here, we investigate the efficiency of SiO2NPs in controlling the American serpentine leafminer Liriomyza trifolii (Diptera: Agromyzidae), a devastating insect pest of a wide range of crops. SiO2NPs at concentrations of 50, 100, 200 and 400 mg/L compared to a control of distilled water were applied to intact Phaseolus vulgaris leaves by spraying to evaluate their effects, via the plant, on the number, survival, developmental/feeding speed, body mass/size and sex ratio of L. trifolii released after spraying. A qRT-PCR analysis was conducted to assess oxidative stress in L. trifolii based on the gene expression level of the two major antioxidant enzymes; catalase and superoxide dismutase. The number of mines, survival rate and puparia weight were lower than the control at the highest concentration of SiO2NPs, while pupariation was delayed at most of the concentrations studied and puparium weight/size decreased at low concentrations. Sex ratio was not affected by SiO2NPs. Gene expression levels of the antioxidant enzymes at the pupal stage were not significantly affected by SiO2NPs at any concentration but some individuals showed up-regulation at low SiO2NP concentrations, inferring development of resistance. This study demonstrated that a priori application of the highest concentration of SiO2NPs to plants effectively reduces a leafminer population but that application of even the lowest concentration can decrease the life history trait values of surviving leafminers.

Subject Areas

Silica nanoparticles; Liriomyza trifolii; Phaseolus vulgaris; qRT-PCR; Oxidative stress; Dose dependency

Comments (1)

Comment 1
Received: 7 December 2020
Commenter: Midori Tuda
Commenter's Conflict of Interests: Author
Comment: Additional data of the leafminer (number of mines, mine appearance date, pupariation date, puparium size and adult size). Modification of the title and text.
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