Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Aging Induces Profound Changes in sncRNA in Rat Sperm and these Changes are Modified by Perinatal Exposure to Environmental Flame Retardant

Alexander Suvorov
* ORCID logo ,
Vladimir Naumov
,
Victoria Shtratnikova
ORCID logo ,
J.Richard Pilsner
,
Anna Zheludkevich
,
Evgeny Gerasimov
ORCID logo ,
Maria Logacheva
,
Oleg Sergeyev
ORCID logo
Version 1 : Received: 15 October 2020 / Approved: 16 October 2020 / Online: 16 October 2020 (08:10:16 CEST)

A peer-reviewed article of this Preprint also exists.

Suvorov, A.; Pilsner, J.R.; Naumov, V.; Shtratnikova, V.; Zheludkevich, A.; Gerasimov, E.; Logacheva, M.; Sergeyev, O. Aging Induces Profound Changes in sncRNA in Rat Sperm and These Changes Are Modified by Perinatal Exposure to Environmental Flame Retardant. Int. J. Mol. Sci. 2020, 21, 8252. Suvorov, A.; Pilsner, J.R.; Naumov, V.; Shtratnikova, V.; Zheludkevich, A.; Gerasimov, E.; Logacheva, M.; Sergeyev, O. Aging Induces Profound Changes in sncRNA in Rat Sperm and These Changes Are Modified by Perinatal Exposure to Environmental Flame Retardant. Int. J. Mol. Sci. 2020, 21, 8252.

Abstract

Advanced paternal age at fertilization is a risk factor for multiple disorders in offspring and may be linked with age-related epigenetic changes in fathers sperm. Understanding of aging-related epigenetic changes in sperm and environmental factors that modify such changes is needed. Here we characterize changes in sperm sncRNA between young pubertal and mature rats. We also analyze modification of these changes by exposure to environmental xenobiotic 2,2’,4,4’-tetrabromodiphenyl ether (BDE-47). SncRNA libraries prepared from epididymal spermatozoa were sequenced and analyzed using DESeq 2. Distribution of small RNA fractions changed with age, with fractions mapping to rRNA and lncRNA decreasing and fractions mapping to tRNA and miRNA increasing. 249 miRNA, 908 piRNA and 227 tRNA-derived RNA were differentially expressed (2-fold change, FDR p ≤ 0.05) between age groups in control animals. Differentially expressed miRNA and piRNA were enriched for protein-coding targets involved in development and metabolism, piRNA were enriched for LTR targets. BDE-47 accelerated age dependent changes in sncRNA in younger animals, decelerated these changes in older animals and increased the variance in expression of all sncRNA. Our results indicate that the natural aging process has profound effects on sperm sncRNA profiles and this effect may be modified by environmental exposures.

Keywords

aging; paternal exposure; sperm; semen; epigenetics; sncRNA; piRNA; miRNA; 2,2′,4,4′-tetrabromodiphenyl ether; PBDE; BDE-47; perinatal; environment.

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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