Working Paper Article Version 3 This version is not peer-reviewed

Exogenous H2S Regulates the Expression of Cystathionine Gamma-Lyase in HUVECs During Hypoxia

Version 1 : Received: 10 October 2020 / Approved: 14 October 2020 / Online: 14 October 2020 (13:10:40 CEST)
Version 2 : Received: 28 May 2021 / Approved: 28 May 2021 / Online: 28 May 2021 (11:54:51 CEST)
Version 3 : Received: 17 June 2021 / Approved: 23 June 2021 / Online: 23 June 2021 (11:51:08 CEST)

How to cite: Wang, M. Exogenous H2S Regulates the Expression of Cystathionine Gamma-Lyase in HUVECs During Hypoxia. Preprints 2020, 2020100307 Wang, M. Exogenous H2S Regulates the Expression of Cystathionine Gamma-Lyase in HUVECs During Hypoxia. Preprints 2020, 2020100307

Abstract

Cystathionine gamma-lyase (CSE) is an essential Hydrogen sulphide (H2S)-producing enzyme that regulates diverse processes related to cardiovascular function. It is crucial to understand how exogenous H2S regulates CSE expression in vascular endothelial cells during hypoxia. We examined the regulatory effect of 100 μM H2S on the transcription and expression of CSE in HUVECs during hypoxia by luciferase assay, Western blotting, and quantitative RT-qPCR. Exogenous H2S influenced the promoter activity of CSE in HUVECs during hypoxia. Compared with 0 μM H2S, 100 μM H2S decreased the mRNA expression of CSE in HUVECs. Compared with 0 μM H2S, 100 μM H2S decreased the protein expression of CSE in HUVECs after 2 h of hypoxia. These findings suggest that vascular endothelial cells can respond to changes in H2S concentration in the blood during hypoxia.

Keywords

Cystathionine gamma-lyase; Hydrogen sulfide; Hypoxia; HUVECs

Comments (1)

Comment 1
Received: 23 June 2021
Commenter: Maoxian Wang
Commenter's Conflict of Interests: Author
Comment: The edtion of manuscript has been revised with language by the AJE website.
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