Working Paper Article Version 2 This version is not peer-reviewed

Exogenous H2S Regulates the Expression of Cystathionine Gamma-Lyase In HUVECs During Hypoxia

Version 1 : Received: 10 October 2020 / Approved: 14 October 2020 / Online: 14 October 2020 (13:10:40 CEST)
Version 2 : Received: 28 May 2021 / Approved: 28 May 2021 / Online: 28 May 2021 (11:54:51 CEST)
Version 3 : Received: 17 June 2021 / Approved: 23 June 2021 / Online: 23 June 2021 (11:51:08 CEST)

How to cite: Wang, M. Exogenous H2S Regulates the Expression of Cystathionine Gamma-Lyase In HUVECs During Hypoxia. Preprints 2020, 2020100307 Wang, M. Exogenous H2S Regulates the Expression of Cystathionine Gamma-Lyase In HUVECs During Hypoxia. Preprints 2020, 2020100307

Abstract

Cystathionine gamma-lyase (CSE) is one of the essential H2S-producing enzymes, and it regulates diverse functions in connection with cardiovascular function. It is crucial how exogenous H2S regulates CSE expression of the vascular endothelial cell during hypoxia. We examined the transcription and expression of CSE in HUVECs regulated by exogenous H2S with 100 μM during hypoxia by Luciferase assay, Western blotting, and quantitative RT-qPCR. Exogenous H2S influenced the promoter activity of CSE in HUVECs during hypoxia. The effects of 100 μM H2S on CSE mRNA expression in HUVECs is decreased compared with 0 μM H2S. The consequences of 100 μM H2S on the expression level of CSE protein in HUVECs at 2 h of hypoxia is reduced compared with 0 μM H2S. These findings suggest that vascular endothelial cells can respond to changes in H2S concentration in the blood during hypoxia.

Keywords

Cystathionine gamma-lyase; Hydrogen sulfide; Hypoxia; HUVECs

Comments (1)

Comment 1
Received: 28 May 2021
Commenter: Maoxian Wang
Commenter's Conflict of Interests: Author
Comment: Some modifications have been made to the conclusion of the article, as well as the grammar and other aspects.Changes have been made to the legend description.
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