Watanabe, T.; Hayashi, K.; Takahashi, I.; Ohwaki, M.; Kan, T.; Kawahara, T. Enhanced Protection Against Influenza Virus Infection with Dispersion Controlled Lactic Acid Bacteria Powder. Preprints2020, 2020100242. https://doi.org/10.20944/preprints202010.0242.v1
APA Style
Watanabe, T., Hayashi, K., Takahashi, I., Ohwaki, M., Kan, T., & Kawahara, T. (2020). Enhanced Protection Against Influenza Virus Infection with Dispersion Controlled Lactic Acid Bacteria Powder. Preprints. https://doi.org/10.20944/preprints202010.0242.v1
Chicago/Turabian Style
Watanabe, T., Tatsuhiko Kan and Toshio Kawahara. 2020 "Enhanced Protection Against Influenza Virus Infection with Dispersion Controlled Lactic Acid Bacteria Powder" Preprints. https://doi.org/10.20944/preprints202010.0242.v1
Abstract
We evaluated the change in water dispersibility of lactic acid bacteria (LAB, Enterococcus faecalis KH2) upon powderization and its influence on their efficacy. When cultured LAB are washed, heat-killed, and powdered, adhesion between LAB occurs and they form aggregation (non-treated LAB, n-LAB). However, a dispersed LAB (d-LAB) powder with less aggregates can be prepared by treating them with a high-pressure homogenizer and adding an excipient during powdering. n-LAB or d-LAB was administered to mice and the Peyer's patches in the small intestine were observed. n-LAB administration showed a high amount of aggregated LAB drifting in the intestinal mucosa, whereas d-LAB reached the Peyer's patches and was taken up into the Peyer's patches. Evaluation in a mouse influenza virus infection model showed that d-LAB was more effective than n-LAB in influenza yield of bronchoalveolar lavage fluids (BALFs) on day 3 post-infection, neutralizing antibody titers of sera and influenza virus-specific IgA in the feces on day 14 post-infection. Thus, the physical properties of LAB affect their efficacy; controlling their water dispersibility can improve their effectiveness.
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