Working Paper Article Version 1 This version is not peer-reviewed

Histomorphometric, Immunohistochemical, Ultrastructural and Statistic Biomaterial Evaluation: Comparison between Synthetic Nano-Hydroxyapatite /Beta-Tricalcium Phosphate and Hydroxyapatite in No Critic Defects in Rat Calvaria

Version 1 : Received: 4 October 2020 / Approved: 6 October 2020 / Online: 6 October 2020 (13:18:42 CEST)

A peer-reviewed article of this Preprint also exists.

da Silva Brum, I.; Frigo, L.; Lana Devita, R.; da Silva Pires, J.L.; Hugo Vieira de Oliveira, V.; Rosa Nascimento, A.L.; de Carvalho, J.J. Histomorphometric, Immunohistochemical, Ultrastructural Characterization of a Nano-Hydroxyapatite/Beta-Tricalcium Phosphate Composite and a Bone Xenograft in Sub-Critical Size Bone Defect in Rat Calvaria. Materials 2020, 13, 4598. da Silva Brum, I.; Frigo, L.; Lana Devita, R.; da Silva Pires, J.L.; Hugo Vieira de Oliveira, V.; Rosa Nascimento, A.L.; de Carvalho, J.J. Histomorphometric, Immunohistochemical, Ultrastructural Characterization of a Nano-Hydroxyapatite/Beta-Tricalcium Phosphate Composite and a Bone Xenograft in Sub-Critical Size Bone Defect in Rat Calvaria. Materials 2020, 13, 4598.

Journal reference: Materials 2020, 13, 4598
DOI: 10.3390/ma13204598

Abstract

Nowadays, we can observe a worldwide trend towards the development of synthetic biomaterials. Numerous researches have been carried out in order to better understand the cellular behavior involved in the inflammation and bone healing processes related to living tissues. The aim of this study is to evaluate the tissue behavior of two different types of biomaterials: synthetic nano-hydroxyapatite / beta-tricalcium phosphate and hydroxyapatite in non-critical bone defects in rat calvaria. Twenty four rats underwent surgery in which two 3 mm defects in each cavity were performed. The rats were divided into 2 groups: Group 1, using xenogen hydroxyapatite (Bio oss ™); Group 2, using synthetic nano-hydroxyapatite / beta-tricalcium phosphate (Blue Bone ™). Sixty days after the surgery, the calvaria bone defect was filled with biomaterial, the animals were sacrificed and marked with Masson's trichrome and PAS staining, and immunohistochemistry (TNF-α, MMP-9) and electron microscopy analyzes were performed. Histomorphometric analysis indicates a greater presence in the protein matrix in Group 2, in addition to higher levels of TNF-α, MMP-9. The ultrastructural analysis shows an association of material with fibroblasts in the tissue regeneration stage. Paired statistical data indicates that Blue Bone ™ can improve bone formation / remodeling when compared to biomaterials of xenogenous origin.

Subject Areas

synthetic; xenogenous; nano-hydroxyapatita; beta-tricalcium phosphate

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