Version 1
: Received: 21 September 2020 / Approved: 22 September 2020 / Online: 22 September 2020 (09:43:55 CEST)
How to cite:
Shakeela, Q.; Liaqat, S.; Ali, F.; Niaz, Z.; Hayat, A.; Rehman, M.; Ahmed, S. Molecular Identification of Quorum Sensing and Quorum Quenching Bacteria Isolated from Soil and Hospital Setup. Preprints2020, 2020090515
Shakeela, Q.; Liaqat, S.; Ali, F.; Niaz, Z.; Hayat, A.; Rehman, M.; Ahmed, S. Molecular Identification of Quorum Sensing and Quorum Quenching Bacteria Isolated from Soil and Hospital Setup. Preprints 2020, 2020090515
Shakeela, Q.; Liaqat, S.; Ali, F.; Niaz, Z.; Hayat, A.; Rehman, M.; Ahmed, S. Molecular Identification of Quorum Sensing and Quorum Quenching Bacteria Isolated from Soil and Hospital Setup. Preprints2020, 2020090515
APA Style
Shakeela, Q., Liaqat, S., Ali, F., Niaz, Z., Hayat, A., Rehman, M., & Ahmed, S. (2020). Molecular Identification of Quorum Sensing and Quorum Quenching Bacteria Isolated from Soil and Hospital Setup. Preprints. https://doi.org/
Chicago/Turabian Style
Shakeela, Q., Mujadda-Ur- Rehman and Shehzad Ahmed. 2020 "Molecular Identification of Quorum Sensing and Quorum Quenching Bacteria Isolated from Soil and Hospital Setup" Preprints. https://doi.org/
Abstract
N-Acyl-homoserine lactones, the Quorum sensing signaling molecules predominantly found in gram-negative bacteria, which regulate several bacterial genes including virulence and antibiotic resistant genes. The study was aimed to identify and characterize QS and QQ bacteria from different samples. 5 samples with different ecological background were collected from soil and 10 samples from hospital setup. 31 different bacteria were isolated with either QS or QQ activities all together. CV026 and A136 biosensor strains were used for the detection of QS and QQ positive strains. QS activity was observed by cross streaking test of bacteria against CV026, it was affirmed that 13 isolates from the soil and 5 from hospital equipment’s showed positive QS activity. QQ activity of each isolate was tested by well diffusion assay, C6-HSL and C12-HSL were our candidate AHL molecules. The AHL molecule degradation was detected in 4 isolates of soil and none from the samples obtained from hospital setup. The total of 6 strongly positive QS and QQ isolates were identified and selected for 16S rRNA gene sequencing. The phylogenetic analysis revealed that these isolates were closely related to Pseudomonas, Bacillus and Exiguobacterium genera. In contrast, 1 Gram positive bacterial isolate was also purified with QS potential.
Keywords
Quorum sensing; Quorum quenching; AHL molecules; 16S rRNA
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
