Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Focus on Mascarenes Endemic Plants with Specific Phytochemical Composition, Potent Antioxidant and Antiproliferative Properties

Nawraj Rummun
,
Philippe Rondeau
,
Emmanuel Bourdon
ORCID logo ,
Elisabete Pires
,
James McClullagh
,
Timothy D.W. Claridge
,
Theeshan Bahorun
ORCID logo ,
Wen-Wu Li
* ORCID logo ,
Vidushi S. Neergheen
*
Version 1 : Received: 3 September 2020 / Approved: 4 September 2020 / Online: 4 September 2020 (10:28:57 CEST)

How to cite: Rummun, N.; Rondeau, P.; Bourdon, E.; Pires, E.; McClullagh, J.; Claridge, T.D.; Bahorun, T.; Li, W.; Neergheen, V.S. Focus on Mascarenes Endemic Plants with Specific Phytochemical Composition, Potent Antioxidant and Antiproliferative Properties. Preprints 2020, 2020090094. https://doi.org/10.20944/preprints202009.0094.v1 Rummun, N.; Rondeau, P.; Bourdon, E.; Pires, E.; McClullagh, J.; Claridge, T.D.; Bahorun, T.; Li, W.; Neergheen, V.S. Focus on Mascarenes Endemic Plants with Specific Phytochemical Composition, Potent Antioxidant and Antiproliferative Properties. Preprints 2020, 2020090094. https://doi.org/10.20944/preprints202009.0094.v1

Abstract

Tropical forests constitute prolific sanctuary of unique floral diversity and potential medicinal sources, however, many of them remains unexplored. Herein, seven Mascarene endemic plants leaves were extracted and evaluated for their in vitro antioxidant properties and antiproliferative effects on a panel of cancer cell lines using MTT and clonogenic cell survival assay. Flow cytometry and comet assay were used to investigate the cell cycle and DNA damaging effects, respectively. Bioassay guided-fractionation coupled with LC-Mass spectrometry (MS), gas chromatography-MS, and nuclear magnetic resonance spectroscopy analysis were used to identify the bioactive compounds. Among the seven plants tested, Terminalia bentzoë was comparatively the most potent antioxidant extract with significantly (p < 0.05) higher cytotoxic activities. T. bentzoë extract further selectively suppressed the growth of human hepatocellular carcinoma cells and significantly halted the cell cycle progression in G0/G1 phase, decreased the cells replicative potential and induced significant DNA damage. Ten phenolic compounds including punicalagin and ellagic acid were identified and likely contributed to the extract potent antioxidant and cytotoxic activities. These results established a promising basis for further in-depth investigations on the potential use of T. bentzoë as supportive therapy in cancer management.

Keywords

Terminalia bentzoë; Mascarene endemic; cytotoxicity; antioxidant; cell cycle arrest; phenolics; bioassay-guided fractionation

Subject

Medicine and Pharmacology, Pharmacology and Toxicology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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