Petrocchi-Rilo, M.; Gutiérrez-Martín, C.-B.; Pérez-Fernández, E.; Vilaró, A.; Fraile, L.; Martínez-Martínez, S. Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern. Antibiotics2020, 9, 614.
Petrocchi-Rilo, M.; Gutiérrez-Martín, C.-B.; Pérez-Fernández, E.; Vilaró, A.; Fraile, L.; Martínez-Martínez, S. Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern. Antibiotics 2020, 9, 614.
Petrocchi-Rilo, M.; Gutiérrez-Martín, C.-B.; Pérez-Fernández, E.; Vilaró, A.; Fraile, L.; Martínez-Martínez, S. Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern. Antibiotics2020, 9, 614.
Petrocchi-Rilo, M.; Gutiérrez-Martín, C.-B.; Pérez-Fernández, E.; Vilaró, A.; Fraile, L.; Martínez-Martínez, S. Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern. Antibiotics 2020, 9, 614.
Abstract
Forty-eight Pasteurella multocida isolates were recovered from porcine pneumonic lungs collected in Norwestern Spain (2017- 2019). These isolates were characterized for their minimal inhibition concentrations to twelve antimicrobial agents and for the appearance of eight resistance genes: tetA, tetB, blaROB1, blaTEM, ermA, ermC, mphE and msrE. Relevant resistance percentages were shown to teracyclines, sulphamethoxazole/trimethoprim and tiamulin, thus suggesting that P. multocida isolates were mostly susceptible to amoxicillin, ceftiofur, enrofloxacin, florfenicol, marbofloxacin and macrolides. 29.2% of isolates were resistant to more than two antimicrobials. The tetracycline resistance genes (tetA and tetB) were detected in 22.9% of the isolates, but none was positive to both simultaneously; blaROB1 and blaTEM genes were found in one third of isolates but both genes were detected simultaneously in only one isolate. ermC gene was observed in 41.7% of isolates, a percentage that decreased until 22.9% for msrE; finally, ermA was harboured by 16.7% and mphE was not found in any of them. Six clusters were established based on hierarchical clustering analysis on antimicrobial susceptibility for the twelve antimicrobials. Generally, it was unable to foresee the antimicrobial susceptibility pattern for each family and the association of each particular isolate inside the clusters established from the presence or absence of the resistance genes analyzed.
Biology and Life Sciences, Immunology and Microbiology
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