Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Antimicrobial Resistance Genes in Porcine Pasteurella Multocida are not Associated with its Antimicrobial Susceptibility Pattern

Version 1 : Received: 2 September 2020 / Approved: 4 September 2020 / Online: 4 September 2020 (07:47:26 CEST)

A peer-reviewed article of this Preprint also exists.

Petrocchi-Rilo, M.; Gutiérrez-Martín, C.-B.; Pérez-Fernández, E.; Vilaró, A.; Fraile, L.; Martínez-Martínez, S. Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern. Antibiotics 2020, 9, 614. Petrocchi-Rilo, M.; Gutiérrez-Martín, C.-B.; Pérez-Fernández, E.; Vilaró, A.; Fraile, L.; Martínez-Martínez, S. Antimicrobial Resistance Genes in Porcine Pasteurella multocida Are Not Associated with Its Antimicrobial Susceptibility Pattern. Antibiotics 2020, 9, 614.

Journal reference: Antibiotics 2020, 9, 614
DOI: 10.3390/antibiotics9090614

Abstract

Forty-eight Pasteurella multocida isolates were recovered from porcine pneumonic lungs collected in Norwestern Spain (2017- 2019). These isolates were characterized for their minimal inhibition concentrations to twelve antimicrobial agents and for the appearance of eight resistance genes: tetA, tetB, blaROB1, blaTEM, ermA, ermC, mphE and msrE. Relevant resistance percentages were shown to teracyclines, sulphamethoxazole/trimethoprim and tiamulin, thus suggesting that P. multocida isolates were mostly susceptible to amoxicillin, ceftiofur, enrofloxacin, florfenicol, marbofloxacin and macrolides. 29.2% of isolates were resistant to more than two antimicrobials. The tetracycline resistance genes (tetA and tetB) were detected in 22.9% of the isolates, but none was positive to both simultaneously; blaROB1 and blaTEM genes were found in one third of isolates but both genes were detected simultaneously in only one isolate. ermC gene was observed in 41.7% of isolates, a percentage that decreased until 22.9% for msrE; finally, ermA was harboured by 16.7% and mphE was not found in any of them. Six clusters were established based on hierarchical clustering analysis on antimicrobial susceptibility for the twelve antimicrobials. Generally, it was unable to foresee the antimicrobial susceptibility pattern for each family and the association of each particular isolate inside the clusters established from the presence or absence of the resistance genes analyzed.

Subject Areas

Pasteurella multocida; antimicrobial resistance genes; antimicrobial susceptibility patterns; swine

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