Version 1
: Received: 27 August 2020 / Approved: 28 August 2020 / Online: 28 August 2020 (11:33:23 CEST)
How to cite:
Almengló, C.; Caamaño, P.; Fraga, M.; Devesa, J.; Costoya, J.A.; Arce, V.M. From Neural Stem Cells (NSC) to Glioblastoma (GBM): A Natural History of GBM Recapitulated in Vitro. Preprints2020, 2020080637. https://doi.org/10.20944/preprints202008.0637.v1
Almengló, C.; Caamaño, P.; Fraga, M.; Devesa, J.; Costoya, J.A.; Arce, V.M. From Neural Stem Cells (NSC) to Glioblastoma (GBM): A Natural History of GBM Recapitulated in Vitro. Preprints 2020, 2020080637. https://doi.org/10.20944/preprints202008.0637.v1
Almengló, C.; Caamaño, P.; Fraga, M.; Devesa, J.; Costoya, J.A.; Arce, V.M. From Neural Stem Cells (NSC) to Glioblastoma (GBM): A Natural History of GBM Recapitulated in Vitro. Preprints2020, 2020080637. https://doi.org/10.20944/preprints202008.0637.v1
APA Style
Almengló, C., Caamaño, P., Fraga, M., Devesa, J., Costoya, J.A., & Arce, V.M. (2020). From Neural Stem Cells (NSC) to Glioblastoma (GBM): A Natural History of GBM Recapitulated in Vitro. Preprints. https://doi.org/10.20944/preprints202008.0637.v1
Chicago/Turabian Style
Almengló, C., José A. Costoya and Víctor M. Arce. 2020 "From Neural Stem Cells (NSC) to Glioblastoma (GBM): A Natural History of GBM Recapitulated in Vitro" Preprints. https://doi.org/10.20944/preprints202008.0637.v1
Abstract
Due to its aggressive and invasive nature glioblastoma (GBM), the most common and aggressive primary brain tumour in adults, remains almost invariably lethal. Significant advances in the last several years have elucidated much of the molecular and genetic complexities of GBM. However, GBM exhibits a vast genetic variation and a wide diversity of phenotypes that has complicated the development of effective therapeutic strategies. This complex pathogenesis makes it necessary the development of experimental models that could be used to further understand the disease, and also to provide a more realistic testing ground for potential therapies. In this report, we describe the process of transformation of primary mouse embryo astrocytes into immortalized cultures with neural stem cell characteristics, that are able to generate of GBM when injected in the brain of C57BL/6 mice, or heterotopic tumours when injected iv. Overall, our results show that oncogenic transformation is a fate for NSC if cultured for long periods in vitro. In addition, since no additional hit is necessary to induce the oncogenic transformation, our model may be used to investigate the pathogenesis of gliomagenesis and to test the effectiveness of different drugs throughout the natural history of GBM.
Medicine and Pharmacology, Oncology and Oncogenics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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