PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Leptospermum scoparium (Mānuka) and Cryptomeria japonica (Sugi) Leaf Essential Oil Seasonal Chemical Variation and Their Effect on Antimicrobial Activity
Version 1
: Received: 26 August 2020 / Approved: 27 August 2020 / Online: 27 August 2020 (14:13:24 CEST)
How to cite:
Bang, K.W.; Lewis, G.; Villas-Boas, S.G. Leptospermum scoparium (Mānuka) and Cryptomeria japonica (Sugi) Leaf Essential Oil Seasonal Chemical Variation and Their Effect on Antimicrobial Activity. Preprints2020, 2020080623. https://doi.org/10.20944/preprints202008.0623.v1
Bang, K.W.; Lewis, G.; Villas-Boas, S.G. Leptospermum scoparium (Mānuka) and Cryptomeria japonica (Sugi) Leaf Essential Oil Seasonal Chemical Variation and Their Effect on Antimicrobial Activity. Preprints 2020, 2020080623. https://doi.org/10.20944/preprints202008.0623.v1
Bang, K.W.; Lewis, G.; Villas-Boas, S.G. Leptospermum scoparium (Mānuka) and Cryptomeria japonica (Sugi) Leaf Essential Oil Seasonal Chemical Variation and Their Effect on Antimicrobial Activity. Preprints2020, 2020080623. https://doi.org/10.20944/preprints202008.0623.v1
APA Style
Bang, K.W., Lewis, G., & Villas-Boas, S.G. (2020). <em>Leptospermum scoparium</em> (Mānuka) and <em>Cryptomeria japonica</em> (Sugi) Leaf Essential Oil Seasonal Chemical Variation and Their Effect on Antimicrobial Activity. Preprints. https://doi.org/10.20944/preprints202008.0623.v1
Chicago/Turabian Style
Bang, K.W., Gillian Lewis and Silas G. Villas-Boas. 2020 "<em>Leptospermum scoparium</em> (Mānuka) and <em>Cryptomeria japonica</em> (Sugi) Leaf Essential Oil Seasonal Chemical Variation and Their Effect on Antimicrobial Activity" Preprints. https://doi.org/10.20944/preprints202008.0623.v1
Abstract
This study evaluated the antimicrobial activity of Leptospermum scoparium (Mānuka) and Cryptomeria japonica (Sugi) essential oils and assessed the effect of seasonal chemical variation on the oils’ antimicrobial efficacies. Plate based assays were conducted to elucidate the oils’ spectrum of in vitro antimicrobial activity and to determine the oils’ minimum inhibitory concentrations (MIC) as a measure of antimicrobial efficacy. Gas chromatography – mass spectrometry was adopted to chemically profile oils distilled in different seasons. The resultant compositional information in conjunction with MIC data was used to evaluate the effect of seasonal variation on the oils’ antimicrobial efficacy. Both Mānuka and Sugi essential oils were active against all classes of target microorganisms. However, limited activity was observed against Gram-negative bacteria. The oils displayed consistent chemotypic characteristics regardless of the time of distillation. Nonetheless, there were quantitative differences in compound abundance in both essential oils. Significant differences in the MIC of Sugi essential oil was observed against target microorganisms as a result of seasonal variation in constituent abundances while Mānuka essential oil’s antimicrobial efficacy was unaffected. This study demonstrates that seasonal chemical variation is an important quality assurance parameter to consider for future application of essential oils as antimicrobial agents in consumer products.
Keywords
Leptospermum scoparium; Cryptomeria japonica; Mānuka; Sugi; Essential oils; Natural products; Secondary metabolites; Antimicrobials; Gas chromatography – Mass spectrometry
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
