Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Circulating RP11-445H22.4 and TINCR are Potential Biomarkers for Invasive Ductal Carcinoma of the Breast

Version 1 : Received: 26 August 2020 / Approved: 27 August 2020 / Online: 27 August 2020 (08:58:55 CEST)

How to cite: Moradi, M.; Rahimi, Z. Circulating RP11-445H22.4 and TINCR are Potential Biomarkers for Invasive Ductal Carcinoma of the Breast. Preprints 2020, 2020080605 (doi: 10.20944/preprints202008.0605.v1). Moradi, M.; Rahimi, Z. Circulating RP11-445H22.4 and TINCR are Potential Biomarkers for Invasive Ductal Carcinoma of the Breast. Preprints 2020, 2020080605 (doi: 10.20944/preprints202008.0605.v1).

Abstract

Purpose: Recently introduced molecules namely long non-coding RNAs (lncRNAs) are associated with various diseases, including breast cancer, and they may have the potential to act as biomarkers. Invasive ductal carcinoma (IDC) is a frequently observed type of breast cancer that diagnosis through immunohistochemical methods. However, no reliable non-invasive markers such as circulating markers have been introduced for it, so far. Materials and methods: The relative quantitation (RQ) of two lncRNAs, RP11-445H22.4 and TINCR, was measured in tumoral tissues and paired adjacent non-tumoral tissues (PANTs) as well as in plasma samples using real-time polymerase chain reaction (RT-PCR). Furthermore, promoter methylation of the two lncRNAs genes was assessed using methylation-specific PCR (MSP). In order to compare the diagnostic values, plasma levels of CA 15-3 were measured as a conventional circulating biomarker using ELISA. The receiver operating characteristic (ROC) curve was used to illustrate the sensitivity and specificity of the potential biomarkers. Results: The RP11-445H22.4 and TINCR genes were upregulated in tumor tissues compared to PANTs (RQRP11-445H22.4 = 2.7, RQTINCR = 4.4). Furthermore, the presence of RP11-445H22.4 and TINCR in the plasma of patients was significantly more than healthy controls (RQc.RP11-445H22.4 = 4.12, RQc.TINCR = 4.16, P<0.001). The promoters of RP11-445H22.4 and TINCR genes were hypomethylated in the tumoral tissues with a negative correlation with their gene expression. Conclusion: Based on the findings, it can be speculated that the expression levels of these two lncRNAs as well as their promoter methylation can be considered as a therapeutic target and potential biomarker for breast carcinoma.

Subject Areas

biomarker; breast cancer; RP11-445H22.4; lncRNA; TINCR

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