Version 1
: Received: 26 August 2020 / Approved: 27 August 2020 / Online: 27 August 2020 (08:58:55 CEST)
How to cite:
Moradi, M.; Rahimi, Z. Circulating RP11-445H22.4 and TINCR are Potential Biomarkers for Invasive Ductal Carcinoma of the Breast. Preprints2020, 2020080605. https://doi.org/10.20944/preprints202008.0605.v1
Moradi, M.; Rahimi, Z. Circulating RP11-445H22.4 and TINCR are Potential Biomarkers for Invasive Ductal Carcinoma of the Breast. Preprints 2020, 2020080605. https://doi.org/10.20944/preprints202008.0605.v1
Moradi, M.; Rahimi, Z. Circulating RP11-445H22.4 and TINCR are Potential Biomarkers for Invasive Ductal Carcinoma of the Breast. Preprints2020, 2020080605. https://doi.org/10.20944/preprints202008.0605.v1
APA Style
Moradi, M., & Rahimi, Z. (2020). Circulating RP11-445H22.4 and TINCR are Potential Biomarkers for Invasive Ductal Carcinoma of the Breast. Preprints. https://doi.org/10.20944/preprints202008.0605.v1
Chicago/Turabian Style
Moradi, M. and Zohreh Rahimi. 2020 "Circulating RP11-445H22.4 and TINCR are Potential Biomarkers for Invasive Ductal Carcinoma of the Breast" Preprints. https://doi.org/10.20944/preprints202008.0605.v1
Abstract
Purpose: Recently introduced molecules namely long non-coding RNAs (lncRNAs) are associated with various diseases, including breast cancer, and they may have the potential to act as biomarkers. Invasive ductal carcinoma (IDC) is a frequently observed type of breast cancer that diagnosis through immunohistochemical methods. However, no reliable non-invasive markers such as circulating markers have been introduced for it, so far. Materials and methods: The relative quantitation (RQ) of two lncRNAs, RP11-445H22.4 and TINCR, was measured in tumoral tissues and paired adjacent non-tumoral tissues (PANTs) as well as in plasma samples using real-time polymerase chain reaction (RT-PCR). Furthermore, promoter methylation of the two lncRNAs genes was assessed using methylation-specific PCR (MSP). In order to compare the diagnostic values, plasma levels of CA 15-3 were measured as a conventional circulating biomarker using ELISA. The receiver operating characteristic (ROC) curve was used to illustrate the sensitivity and specificity of the potential biomarkers. Results: The RP11-445H22.4 and TINCR genes were upregulated in tumor tissues compared to PANTs (RQRP11-445H22.4 = 2.7, RQTINCR = 4.4). Furthermore, the presence of RP11-445H22.4 and TINCR in the plasma of patients was significantly more than healthy controls (RQc.RP11-445H22.4 = 4.12, RQc.TINCR = 4.16, P<0.001). The promoters of RP11-445H22.4 and TINCR genes were hypomethylated in the tumoral tissues with a negative correlation with their gene expression. Conclusion: Based on the findings, it can be speculated that the expression levels of these two lncRNAs as well as their promoter methylation can be considered as a therapeutic target and potential biomarker for breast carcinoma.
Keywords
biomarker; breast cancer; RP11-445H22.4; lncRNA; TINCR
Subject
Medicine and Pharmacology, Oncology and Oncogenics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
