Working Paper Communication Version 2 This version is not peer-reviewed

Mixed Amphiphilic Polymeric Nanoparticles of Chitosan, Poly(Vinyl Alcohol) and Poly(Methyl Methacrylate) for Intranasal Drug Delivery: A Preliminary in Vivo Study

Version 1 : Received: 5 August 2020 / Approved: 6 August 2020 / Online: 6 August 2020 (11:22:55 CEST)
Version 2 : Received: 22 September 2020 / Approved: 23 September 2020 / Online: 23 September 2020 (04:23:41 CEST)

A peer-reviewed article of this Preprint also exists.

Schlachet, I.; Moshe Halamish, H.; Sosnik, A. Mixed Amphiphilic Polymeric Nanoparticles of Chitosan, Poly(vinyl alcohol) and Poly(methyl methacrylate) for Intranasal Drug Delivery: A Preliminary In Vivo Study. Molecules 2020, 25, 4496. Schlachet, I.; Moshe Halamish, H.; Sosnik, A. Mixed Amphiphilic Polymeric Nanoparticles of Chitosan, Poly(vinyl alcohol) and Poly(methyl methacrylate) for Intranasal Drug Delivery: A Preliminary In Vivo Study. Molecules 2020, 25, 4496.

Journal reference: Molecules 2020, 25, 4496
DOI: 10.3390/molecules25194496

Abstract

Intranasal (i.n.) administration became an alternative strategy to bypass the blood-brain barrier and improve drug bioavailability in the brain. The main goal of this work was to preliminarily study the biodistribution of mixed amphiphilic mucoadhesive nanoparticles made of chitosan-g-poly(methyl methacrylate) and poly(vinyl alcohol)-g-poly(methyl methacrylate) and ionotropically crosslinked with sodium tripolyphosphate in the brain after intravenous (i.v.) and i.n. administration to Hsd:ICR mice. After i.v. administration, the highest nanoparticle accumulation was detected in the liver, among other peripheral organs. After i.n. administration of a 10-times smaller nanoparticle dose, the accumulation of the nanoparticles in off-target organs was much lower than after i.v. injection. In particular, the accumulation of the nanoparticles in the liver was 20 times lower than by i.v. When brains were analyzed separately, intravenously administered nanoparticles accumulated mainly in the “top” brain, reaching a maximum after 1h. Conversely, in i.n. administration, nanoparticles were detected in the “bottom” brain and the head (maximum reached after 2 h) owing to their retention in the nasal mucosa and could serve as a reservoir from which the drug is released and transported to the brain over time. Overall results indicate that i.n. nanoparticles reach similar brain bioavailability, though with a 10-fold smaller dose, and accumulate in off-target organs to a more limited extent and only after redistribution through the systemic circulation. At the same time, both administration routes seem to lead to differential accumulation in brain regions and thus, they could be beneficial in the treatment of different medical conditions.

Subject Areas

Central nervous system (CNS); blood-brain barrier (BBB); self-assembled polymeric nanoparticles; intranasal delivery; biodistribution

Comments (1)

Comment 1
Received: 23 September 2020
Commenter: ALEJANDRO SOSNIK
Commenter's Conflict of Interests: Author
Comment: This is a revised version after peer review in the journal Molecules
+ Respond to this comment

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 1
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.