Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Long noncoding RNA PVT1 is Regulated by Bromodomain Protein BRD4 in Multiple Myeloma and is Associated with Disease Progression

Hiroshi Handa
* ORCID logo ,
Kazuki Honma
,
Tsukasa Oda
,
Nobuhiko Kobayashi
,
Yuko Kuroda
,
Kei Kimura-Masuda
,
Saki Watanabe
,
Rei Ishihara
,
Yuki Murakami
,
Yuta Masuda
,
Ken-ichi Tahara
,
Hisashi Takei
,
Tetsuhiro Kasamatsu
,
Takayuki Saitoh
,
Hirokazu Murakami
Version 1 : Received: 3 August 2020 / Approved: 4 August 2020 / Online: 4 August 2020 (11:31:37 CEST)

A peer-reviewed article of this Preprint also exists.

Handa, H.; Honma, K.; Oda, T.; Kobayashi, N.; Kuroda, Y.; Kimura-Masuda, K.; Watanabe, S.; Ishihara, R.; Murakami, Y.; Masuda, Y.; Tahara, K.-I.; Takei, H.; Kasamatsu, T.; Saitoh, T.; Murakami, H. Long Noncoding RNA PVT1 Is Regulated by Bromodomain Protein BRD4 in Multiple Myeloma and Is Associated with Disease Progression. Int. J. Mol. Sci. 2020, 21, 7121. Handa, H.; Honma, K.; Oda, T.; Kobayashi, N.; Kuroda, Y.; Kimura-Masuda, K.; Watanabe, S.; Ishihara, R.; Murakami, Y.; Masuda, Y.; Tahara, K.-I.; Takei, H.; Kasamatsu, T.; Saitoh, T.; Murakami, H. Long Noncoding RNA PVT1 Is Regulated by Bromodomain Protein BRD4 in Multiple Myeloma and Is Associated with Disease Progression. Int. J. Mol. Sci. 2020, 21, 7121.

Abstract

Abstract: Long noncoding RNAs (lncRNAs) are deregulated in human cancers and are associated with disease progression. Plasmacytoma Variant Translocation 1 (PVT1), an lncRNA, is located adjacent to MYC, linked to multiple myeloma (MM). PVT1 is expressed in MM and is associated with carcinogenesis, however, its role and regulation machinery remain uncertain. We examined PVT1/MYC expression through real time PCR in plasma cells purified from 59 MGUS and 140 MM patients. MM cell lines KMS11, KMS12PE, OPM2, and RPMI8226 were treated with JQ1, a MYC superenhancer inhibitor, or MYC inhibitor 10058-F4. The expression levels of PVT1 and MYC were significantly higher in MM than in MGUS (p < 0.0001), and showed positive correlation with disease progression (r = 0.394, p < 0.0001). JQ1 inhibited cell proliferation and decreased the expression levels of MYC and PVT1. However, 10054-F4 did not alter the expression level of PVT1. The positive correlation between MYC and PVT1 in patients, synchronous downregulation of MYC and PVT1 by JQ1, and no effect of MYC inhibitor on PVT1 expression suggest that the expression of these two genes is coregulated by a superenhancer. Cooperative effects between these two genes may contribute to MM pathogenesis and progression.

Keywords

long noncoding RNA; PVT1; MYC; bromodomain; multiple myeloma

Subject

Medicine and Pharmacology, Oncology and Oncogenics

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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