Version 1
: Received: 23 July 2020 / Approved: 24 July 2020 / Online: 24 July 2020 (14:25:19 CEST)
Version 2
: Received: 15 April 2021 / Approved: 16 April 2021 / Online: 16 April 2021 (11:23:51 CEST)
How to cite:
Bryant, P.J.; Arehart, T. Diversity and Life-Cycle Analysis of Pacific Ocean Zooplankton by Videomicroscopy and Dna Barcoding: Crustacea. Preprints2020, 2020070594. https://doi.org/10.20944/preprints202007.0594.v2
Bryant, P.J.; Arehart, T. Diversity and Life-Cycle Analysis of Pacific Ocean Zooplankton by Videomicroscopy and Dna Barcoding: Crustacea. Preprints 2020, 2020070594. https://doi.org/10.20944/preprints202007.0594.v2
Bryant, P.J.; Arehart, T. Diversity and Life-Cycle Analysis of Pacific Ocean Zooplankton by Videomicroscopy and Dna Barcoding: Crustacea. Preprints2020, 2020070594. https://doi.org/10.20944/preprints202007.0594.v2
APA Style
Bryant, P.J., & Arehart, T. (2021). Diversity and Life-Cycle Analysis of Pacific Ocean Zooplankton by Videomicroscopy and Dna Barcoding: Crustacea. Preprints. https://doi.org/10.20944/preprints202007.0594.v2
Chicago/Turabian Style
Bryant, P.J. and Timothy Arehart. 2021 "Diversity and Life-Cycle Analysis of Pacific Ocean Zooplankton by Videomicroscopy and Dna Barcoding: Crustacea" Preprints. https://doi.org/10.20944/preprints202007.0594.v2
Abstract
Determining the DNA sequencing of a small element in the mitochondrial DNA (DNA barcoding) makes it possible to easily identify individuals of different larval stages of marine crustaceans without the need for laboratory rearing. It can also be used to construct taxonomic trees, although it is not yet clear to what extent this barcode-based taxonomy reflects more traditional morphological or molecular taxonomy. Collections of zooplankton were made using conventional plankton nets in Newport Bay and the Pacific Ocean near Newport Beach, California (Lat. 33.628342, Long. -117.927933) between May 2013 and January 2020, and individual crustacean specimens were documented by videomicroscopy. Adult crustaceans were collected from solid substrates in the same areas. Specimens were preserved in ethanol and sent to the Canadian Centre for DNA Barcoding at the University of Guelph, Ontario, Canada for sequencing of the COI DNA barcode. From 1042 specimens, 544 COI sequences were obtained falling into 199 Barcode Identification Numbers (BINs), of which 76 correspond to recognized species. The results show the utility of DNA barcoding for matching life-cycle stages as well as for documenting the diversity of this group of organisms.
Keywords
Crustacea; Zooplankton; Plankton; Pacific Ocean; Larvae; DNA barcoding
Subject
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
