Preprint Article Version 1 This version is not peer-reviewed

miR-148b Differentiates Tubular Atrophy/Interstitial Fibrosis Histopathological Stages in IgA Nephropathy

Version 1 : Received: 4 June 2020 / Approved: 5 June 2020 / Online: 5 June 2020 (14:06:53 CEST)

How to cite: Kumar, S.; C, P.; Parameswaran, S.; Shewade, D.; Viswanathan, P.; Ganesh, R. miR-148b Differentiates Tubular Atrophy/Interstitial Fibrosis Histopathological Stages in IgA Nephropathy. Preprints 2020, 2020060049 (doi: 10.20944/preprints202006.0049.v1). Kumar, S.; C, P.; Parameswaran, S.; Shewade, D.; Viswanathan, P.; Ganesh, R. miR-148b Differentiates Tubular Atrophy/Interstitial Fibrosis Histopathological Stages in IgA Nephropathy. Preprints 2020, 2020060049 (doi: 10.20944/preprints202006.0049.v1).

Abstract

IgA nephropathy (IgAN) is one of the most common forms of glomerular disease. It is diagnosed by the dominant or co-dominant IgA deposition in the mesangial region by histopathological examination of kidney biopsy. Kidney biopsy has its own complication and not performed frequently. microRNA (miRNA) is a small RNA, which plays an important role at the post transcriptional level by downregulating mRNAs. We have tried to establish a miRNA based biomarker for IgAN. We quantified miR-148b and let-7b from plasma in IgAN patients and healthy controls. Logistic regression models and receiver operating curve analysis used to analyze the miRNAs quantity and Oxford MEST-C scoring parameters (M- Mesangial hypercellularity, E- Endocapillary hypercellularity, S- Segmental glomerulosclerosis, T- Tubular atrophy/Interstitial fibrosis, C- Crescents). miR-148b and let-7b levels in IgAN were found to be higher by 2.9 and 5.48 times than the healthy controls, respectively. let-7b was positively correlated with complement C3 levels. Similarly, miR-148b was positively correlated with estimated glomerular filtration rate (eGFR) and negatively correlated with S, T, and blood pressure (BP). The sensitivity, specificity, and area under the curve (AUC) of receiver operating characteristic (ROC) for miR-148b against T were 0.87, 0.77, and 0.85, respectively. The threshold value of miR-148b concentration was found to be 8479 to differentiate the severe condition of IgAN. Furthermore, the decrease in miR-148b concentration at a threshold point indicated the progression of the severity of the IgAN. It can also be used to predict the IgAN at an earlier stage.

Subject Areas

IgAN; miR-148b; let-7b; Tubular atrophy; Interstitial fibrosis; MEST-C

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