Genetic Interaction Between the Non-homologous End Joining Factors during B and T Lymphocyte Development: <em>In Vivo</em> Mouse Models

The non-homologous end joining (NHEJ) DNA repair pathway is the main mechanism to repair DNA double strand breaks (DSBs) throughout the whole cell cycle. During NHEJ, core Ku70 and Ku80 subunits form the Ku heterodimer; Ku binds DSBs and promotes the recruitment of accessory factors (e.g., DNA-PKcs, PAXX, Mri) and downstream core factors (XLF, Lig4 and XRCC4). DSBs are induced during the V(D)J recombination in developing B and T lymphocytes to increase the repertoire of B and T cell receptors. Furthermore, DSBs are generated during the class switch recombination (CSR) in mature B lymphocytes, providing distinct effector functions of antibody heavy chain constant regions. The NHEJ is required for both V(D)J recombination and CSR. During the last decades, new NHEJ proteins have been reported, increasing the complexity of the molecular pathway. Multiple in vivo mouse models were generated and characterized to identify specific functions of NHEJ factors in the adaptive immune system. Here, we are summarizing available mouse models lacking one or several NHEJ factors, with a particular focus on early B cell development.

Keywords:

B cell

;

V(D)J recombination

;

mouse model

;

NHEJ

;

DNA repair

Subject:

Biology and Life Sciences - Cell and Developmental Biology

Copyright: This open access article is published under a Creative Commons CC BY 4.0 license, which permit the free download, distribution, and reuse, provided that the author and preprint are cited in any reuse.

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