Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Determination of Knock-Down Time and Level of Toxicity of Panyawan (Tinospora rumphii L.) Leaf Extract against American Cockroach (Periplaneta americana)

Version 1 : Received: 2 May 2020 / Approved: 3 May 2020 / Online: 3 May 2020 (07:27:40 CEST)

How to cite: Pregoner, J.D.; Flores, L.D.; Sison, G.; Tampepe, E.; Ombing, S.P.; Dominguiano, A.; Bangal, S.; Casado, A.; Villanueva, R.A. Determination of Knock-Down Time and Level of Toxicity of Panyawan (Tinospora rumphii L.) Leaf Extract against American Cockroach (Periplaneta americana). Preprints 2020, 2020050028 (doi: 10.20944/preprints202005.0028.v1). Pregoner, J.D.; Flores, L.D.; Sison, G.; Tampepe, E.; Ombing, S.P.; Dominguiano, A.; Bangal, S.; Casado, A.; Villanueva, R.A. Determination of Knock-Down Time and Level of Toxicity of Panyawan (Tinospora rumphii L.) Leaf Extract against American Cockroach (Periplaneta americana). Preprints 2020, 2020050028 (doi: 10.20944/preprints202005.0028.v1).

Abstract

This paper aims to determine the insecticidal activity of Panyawan (Tinospora rumphii) leaf extract against American cockroach (Periplaneta americana). A quantitative experimental research design was employed in the study. Cockroach strains were collected through trapping and hand catch methods. Panyawan leaf was collected and extracted to achieve seven different concentrations. The concentrations and controls were tested against the strains through the standard contact glass jar bioassay. Results revealed that the shortest knock-down time was achieved by the positive control at 1.08 minutes while the Extracts of Panyawan leaf reported a 1.42 to 20.25 minutes knock-down time. Probit Regression reported that the lethal concentration 50 of Panyawan leaf extract was 15.836%. One-Way ANOVA revealed that there is a significant difference in the knock-down times of cockroaches when exposed to different Panyawan leaf extract concentrations, negative and positive controls (p<0.01). Homogeneous subsets derived from Post Hoc Test using Tukey’s Honest Significant Difference reported that there is no significant difference between positive control and Extract 100% (p>0.01).

Subject Areas

Panyawan (Tinospora rumphii); Knock-down time; Experimental; Davao City

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