Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Determination of Knock-Down Time and Level of Toxicity of Panyawan (Tinospora rumphii L.) Leaf Extract against American Cockroach (Periplaneta americana)

Joseph Dave Pregoner
* ORCID logo ,
Li Deve Flores
,
Germae Sison
,
EJ Tampepe
,
Simon Peter Ombing
,
Andrian Dominguiano
,
Sophia Bangal
,
Angelika Casado
,
Regina Annabelle Villanueva
Version 1 : Received: 2 May 2020 / Approved: 3 May 2020 / Online: 3 May 2020 (07:27:40 CEST)

How to cite: Pregoner, J.D.; Flores, L.D.; Sison, G.; Tampepe, E.; Ombing, S.P.; Dominguiano, A.; Bangal, S.; Casado, A.; Villanueva, R.A. Determination of Knock-Down Time and Level of Toxicity of Panyawan (Tinospora rumphii L.) Leaf Extract against American Cockroach (Periplaneta americana). Preprints 2020, 2020050028. https://doi.org/10.20944/preprints202005.0028.v1 Pregoner, J.D.; Flores, L.D.; Sison, G.; Tampepe, E.; Ombing, S.P.; Dominguiano, A.; Bangal, S.; Casado, A.; Villanueva, R.A. Determination of Knock-Down Time and Level of Toxicity of Panyawan (Tinospora rumphii L.) Leaf Extract against American Cockroach (Periplaneta americana). Preprints 2020, 2020050028. https://doi.org/10.20944/preprints202005.0028.v1

Abstract

This paper aims to determine the insecticidal activity of Panyawan (Tinospora rumphii) leaf extract against American cockroach (Periplaneta americana). A quantitative experimental research design was employed in the study. Cockroach strains were collected through trapping and hand catch methods. Panyawan leaf was collected and extracted to achieve seven different concentrations. The concentrations and controls were tested against the strains through the standard contact glass jar bioassay. Results revealed that the shortest knock-down time was achieved by the positive control at 1.08 minutes while the Extracts of Panyawan leaf reported a 1.42 to 20.25 minutes knock-down time. Probit Regression reported that the lethal concentration 50 of Panyawan leaf extract was 15.836%. One-Way ANOVA revealed that there is a significant difference in the knock-down times of cockroaches when exposed to different Panyawan leaf extract concentrations, negative and positive controls (p<0.01). Homogeneous subsets derived from Post Hoc Test using Tukey’s Honest Significant Difference reported that there is no significant difference between positive control and Extract 100% (p>0.01).

Keywords

Panyawan (Tinospora rumphii); Knock-down time; Experimental; Davao City

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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