PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Cytotoxicity and Mitochondrial-Mediated Apoptosis Induced by Ethanolic Leaf Extract of Barleria lupulina Lindl. in Human Leukemia Cells Via Reactive Oxygen Species Generation
Version 1
: Received: 30 April 2020 / Approved: 2 May 2020 / Online: 2 May 2020 (16:07:45 CEST)
How to cite:
Kumari, R.; Kumar, S. Cytotoxicity and Mitochondrial-Mediated Apoptosis Induced by Ethanolic Leaf Extract of Barleria lupulina Lindl. in Human Leukemia Cells Via Reactive Oxygen Species Generation. Preprints2020, 2020050019. https://doi.org/10.20944/preprints202005.0019.v1
Kumari, R.; Kumar, S. Cytotoxicity and Mitochondrial-Mediated Apoptosis Induced by Ethanolic Leaf Extract of Barleria lupulina Lindl. in Human Leukemia Cells Via Reactive Oxygen Species Generation. Preprints 2020, 2020050019. https://doi.org/10.20944/preprints202005.0019.v1
Kumari, R.; Kumar, S. Cytotoxicity and Mitochondrial-Mediated Apoptosis Induced by Ethanolic Leaf Extract of Barleria lupulina Lindl. in Human Leukemia Cells Via Reactive Oxygen Species Generation. Preprints2020, 2020050019. https://doi.org/10.20944/preprints202005.0019.v1
APA Style
Kumari, R., & Kumar, S. (2020). Cytotoxicity and Mitochondrial-Mediated Apoptosis Induced by Ethanolic Leaf Extract of <em>Barleria lupulina</em> Lindl. in Human Leukemia Cells Via Reactive Oxygen Species Generation. Preprints. https://doi.org/10.20944/preprints202005.0019.v1
Chicago/Turabian Style
Kumari, R. and Sanjay Kumar. 2020 "Cytotoxicity and Mitochondrial-Mediated Apoptosis Induced by Ethanolic Leaf Extract of <em>Barleria lupulina</em> Lindl. in Human Leukemia Cells Via Reactive Oxygen Species Generation" Preprints. https://doi.org/10.20944/preprints202005.0019.v1
Abstract
Background: Barleria lupulina Lindl. (Hop-headed) is a small shrub, possess potent anti-inflammatory, analgesic, anti-leukemic, antitumor, anti-hyperglycemic, anti-amoebic, virucidal, diuretic, bactericidal and antibiotic properties. Methods: Cytotoxicity, bioactive assay and genetic analysis of B. lupulina were investigated in the present communication. The leaf extract was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Neutral red uptake (NRU), DNA fragment, reactive oxygen species (ROS) generation, mitochondrial membrane potential (MMP) assay, gene expression analysis and cDNA synthesis to evaluate anti-cancerous potency using cancerous THP-1 cell lines in vitro and in vivo. Results: HPTLC analysis reveals four spots and GC-MS analysis displayed the presence of eleven bioactive compounds among which benzofuranon, hexadecanoic acid, ethyl 9,12,15-octadecatrienoate, and 3,7,11,15-tetramethyl-2-hexadecanoic acid were the most prominent compounds. The ethanolic extract showed significant cytotoxicity (P<0.5) against THP-1 cell line at a concentration of 1mg/mL. The cells were also observed for apoptosis through DNA fragmentation in B. lupulina treated cells. Conclusions: It can be concluded that if the dose range was further refined within the range of 100-1000 µg/mL there could be dose at which the entire population of the THP-1 cell line would be apoptosis induced. The extract induced ROS in the cells after 30 minutes of exposure displaying cytotoxic effects and DNA fragmentation assay.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
