Preprint Article Version 1 This version is not peer-reviewed

Novel Mutations in the S1 domain of COVID 19 Spike Protein of Isolate from Gujarat Origin, Western India

Version 1 : Received: 22 April 2020 / Approved: 24 April 2020 / Online: 24 April 2020 (14:19:02 CEST)

How to cite: Banerjee, A.K.; Begum, F.; Thagriki, D.; Tripathi, P.P.; Ray, U. Novel Mutations in the S1 domain of COVID 19 Spike Protein of Isolate from Gujarat Origin, Western India. Preprints 2020, 2020040450 (doi: 10.20944/preprints202004.0450.v1). Banerjee, A.K.; Begum, F.; Thagriki, D.; Tripathi, P.P.; Ray, U. Novel Mutations in the S1 domain of COVID 19 Spike Protein of Isolate from Gujarat Origin, Western India. Preprints 2020, 2020040450 (doi: 10.20944/preprints202004.0450.v1).

Abstract

Till date there are three full length COVID 19 virus genome sequences available from India. The earlier two were reported from Kerala, Southern India and the more recent one has been reported from Gujarat, western part of India. In this paper we report two novel mutations in the Spike protein sequence of Gujarat’s isolate. These mutations are based on comparison with the original Wuhan sequence. The two mutations have been found to be located just upstream and downstream of the receptor binding domain (RBD). Out of these two one has also been found to affect the secondary structure of the S1 domain. Since both of these mutations lie near the receptor binding domain, they might influence the spike receptor interactions by changing the conformation of the spike protein S1 domain. These mutations are uniquely placed and might be important in the context of vaccine engineering and therapeutic interventions.

Subject Areas

COVID-19; S protein; RBD; S1; S2; Mutation

Comments (2)

Comment 1
Received: 9 May 2020
Commenter: Dr Ande Murali Varaprasad
The commenter has declared there is no conflict of interests.
Comment: Excellent work.

In D614 mutation of Wuhan Aspartic acid is occupyig the position 614. Is it L-Aspartic acid or D-Aspartic aicd.
As Glycine G replaces the Aspartic acid at position 614 in the Spike protein the virus is spreading faster.
Amino acids can occur in L- and D-forms, but only L-forms are used by cells. Every amino acid (except glycine) can occur in two isomeric forms. Is it the reason why G614 is spreading faster.
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Response 1 to Comment 1
Received: 11 May 2020
Commenter: Upasana Ray
The commenter has declared there is no conflict of interests.
Comment: We have not looked into that aspect yet. Thanks for the input.

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