Preprint Brief Report Version 1 This version is not peer-reviewed

Cytoplasmic Increase of Hsp70 Protein: Potential New Biomarker of Early Infiltration of Cutaneous Squamous Cell Carcinoma Arising from Actinic Keratosis

Version 1 : Received: 8 April 2020 / Approved: 9 April 2020 / Online: 9 April 2020 (12:21:19 CEST)

A peer-reviewed article of this Preprint also exists.

Fernández-Guarino, M.; León, J.J.Z.; Farré, A.J.L.; Morales, M.L.G.; Adrada, A.I.S.; Garde, J.B.; Navalon, J.A.A.; Olasolo, P.J. Cytoplasmic Increase in Hsp70 Protein: A Potential New Biomarker of Early Infiltration of Cutaneous Squamous Cell Carcinoma Arising from Actinic Keratosis. Cancers 2020, 12, 1151. Fernández-Guarino, M.; León, J.J.Z.; Farré, A.J.L.; Morales, M.L.G.; Adrada, A.I.S.; Garde, J.B.; Navalon, J.A.A.; Olasolo, P.J. Cytoplasmic Increase in Hsp70 Protein: A Potential New Biomarker of Early Infiltration of Cutaneous Squamous Cell Carcinoma Arising from Actinic Keratosis. Cancers 2020, 12, 1151.

Journal reference: Cancers 2020, 12, 1151
DOI: 10.3390/cancers12051151

Abstract

Background: Cutaneous squamous skin cell carcinoma (SCC) is the second most frequent type of non- melanoma skin cancer and the second cause of death by skin cancer in caucasian population. However, at present it is difficult to predict patients with worst SCC prognosis. Objective: To identify proteins whose expression level could predict SCC infiltration in SCC arising from actinic keratosis (AC). Methods: A total of 20 biopsies of 20 different patients were studied, 10 were from SCC-AK samples and 10 from normal skin. Early infiltrated SCC-AK were selected on histological examination and to determine the expression of proteins fresh skin samples were processed by 2DE-electrophoresis Results: The expression levels of three proteins namely alpha-hemoglobin, heat shock protein (Hsp)-27 and 70 were significantly increased in SCC-AK samples with respect to normal control skin. However, only the expression level of Hsp70 protein positively correlated with the level of SCC-AK dermis infiltration. Immnunohistological examination suggested that the increased expression of Hsp70 proteins seems to mainly occur in the keratinocytes cytoplasm. The increased cytoplasmic Hsp70 expression in SCC-AK was confirmed by Western-blot experiments. Conclusion: Cytoplasmic expression of Hsp70 could be potential biomarker of early infiltration of SCC arising from an AK. Keywords: actinic keratosis, cutaneous squamous cell carcinoma; cytoplasm, skin cancer; heat shock protein.

Subject Areas

actinic keratosis; cutaneous squamous cell carcinoma; cytoplams; skin cancer; heat shock protein

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