Preprint Review Version 1 This version is not peer-reviewed

Status and Contribution of CRISPR/Cas9 Based Gene-Editing System in the Development of a Low-Immunogenic Wheat Variety

Version 1 : Received: 19 February 2020 / Approved: 21 February 2020 / Online: 21 February 2020 (02:14:36 CET)

How to cite: Verma, A.K.; Tiwari, A.; Lionetti, E.; Srivastava, A.; Monachesi, C.; Catassi, G.N.; Gatti, S.; Galeazzi, T.; Catassi, C. Status and Contribution of CRISPR/Cas9 Based Gene-Editing System in the Development of a Low-Immunogenic Wheat Variety. Preprints 2020, 2020020296 (doi: 10.20944/preprints202002.0296.v1). Verma, A.K.; Tiwari, A.; Lionetti, E.; Srivastava, A.; Monachesi, C.; Catassi, G.N.; Gatti, S.; Galeazzi, T.; Catassi, C. Status and Contribution of CRISPR/Cas9 Based Gene-Editing System in the Development of a Low-Immunogenic Wheat Variety. Preprints 2020, 2020020296 (doi: 10.20944/preprints202002.0296.v1).

Abstract

Gluten, a wheat protein, contains epitopes that trigger celiac disease (CD). So far, there is no treatment available for CD other than following a life-long, strict gluten-free diet (GFD). A very low-gluten or gluten-free wheat could provide an alternative treatment to CD. Till date, conventional plant breeding methods are not sufficient to produce celiac-safe wheat. Clustered Regularly Interspaced Short Palindromic Repeats-associated protein 9 (CRISPR/Cas9) is a versatile gene-editing system may efficiently edit the immunogenic gluten protein thereby producing a celiac-safe wheat variety. However, CRISPR/Cas9-mediated genome editing system has not been widely investigated to edit the wheat genome. Published literature available on various scientific platforms, that applied the CRISPR/Cas9 system to edit the wheat genome were explored. Only original research articles were included. Review articles, protocols, scientific presentations, and Ph.D. thesis were excluded. CRISPR/Cas9 is a highly specific gene-editing technology that can be used to efficiently edit the complex hexaploid wheat genome. It targets a specific wheat genome locus, delete an immunogenic gene and replace it with a preferred gene. CRISPR/Cas9 technology could be a breakthrough in providing an alternative treatment for CD. However, further studies are required to efficiently apply this gene-editing technology to develop a celiac-safe wheat variety.

Subject Areas

CRISPR/Cas9; celiac disease; wheat; sgRNA; gluten; low-immunogenic wheat

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