Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Sample Preservation and Plant Sex Prediction in White Guinea Yam (Dioscorea rotundata Poir.) Seedlings

Version 1 : Received: 12 February 2020 / Approved: 14 February 2020 / Online: 14 February 2020 (04:22:20 CET)

How to cite: Asfaw, A.; Agre, P.; Nwachukwu, C.; Olasanmi, B.; Obidiegwu, J.; Nwachukwu, E.; Adebola, P.; De Koeyer, D. Sample Preservation and Plant Sex Prediction in White Guinea Yam (Dioscorea rotundata Poir.) Seedlings. Preprints 2020, 2020020187 (doi: 10.20944/preprints202002.0187.v1). Asfaw, A.; Agre, P.; Nwachukwu, C.; Olasanmi, B.; Obidiegwu, J.; Nwachukwu, E.; Adebola, P.; De Koeyer, D. Sample Preservation and Plant Sex Prediction in White Guinea Yam (Dioscorea rotundata Poir.) Seedlings. Preprints 2020, 2020020187 (doi: 10.20944/preprints202002.0187.v1).

Abstract

Methods for high-quality DNA extraction and knowledge of sex expression and flowering time are essential for applying genomic-assisted breeding and improve the success with hybridization in Guinea yam. A dioecious or monoecious pattern of flowering and sometimes non-flowering is a common phenomenon within and between the Dioscorea species. The flowering in yam plants raised from botanical seeds often takes an extended period, mostly till the first clonal generation after propagation from the tubers. The prolonged process of testing required to identify plant sex and flowering intensity in yam breeding often poses a challenge to realize reduced breeding cycle and apply genomic selection. This study assessed sample preservation methods for DNA quality during extraction and potential of DNA marker to diagnose plant sex at the early seedling stage in white Guinea yam. The predicted sex at the seedling stage was further validated with the visual score for the sex phenotype at the flowering stage. DNA extracted from leaf samples preserved in liquid nitrogen, silica gel, dry ice, and oven drying methods was similar in quality with a high molecular weight than samples stored in ethanol solution. Yam plant sex diagnosis with the DNA marker (sp16) identified a higher proportion of ZW genotypes (female or monoecious phenotypes) than the ZZ genotypes (male phenotype) in the studied materials with 74% prediction accuracy. The results from this study provided valuable insights on suitable sample preservation methods for quality DNA extraction and the potential of DNA marker sp16 to predict sex in white Guinea yam.

Subject Areas

dioecious; DNA quality; flower type; sample preservation method; sex genotype; sex phenotype; visual assay

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.