Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Characterization of Osteoarthritis in a Medial Meniscectomy-Induced Animal Model Using Contrast-Enhanced X-Ray Microtomography

Version 1 : Received: 8 February 2020 / Approved: 9 February 2020 / Online: 9 February 2020 (15:49:10 CET)

A peer-reviewed article of this Preprint also exists.

Sugasawa, T.; Kuji, T.; Aoki, K.; Yanazawa, K.; Takenouchi, A.; Watanabe, M.; Tome, Y.; Takeuchi, Y.; Aita, Y.; Yahagi, N.; Shishikura, Y.; Ono, S.; Yoshida, Y.; Kawakami, Y.; Takekoshi, K. Characterization of Osteoarthritis in a Medial Meniscectomy-Induced Animal Model Using Contrast-Enhanced X-ray Microtomography. Biomedicines 2020, 8, 56. Sugasawa, T.; Kuji, T.; Aoki, K.; Yanazawa, K.; Takenouchi, A.; Watanabe, M.; Tome, Y.; Takeuchi, Y.; Aita, Y.; Yahagi, N.; Shishikura, Y.; Ono, S.; Yoshida, Y.; Kawakami, Y.; Takekoshi, K. Characterization of Osteoarthritis in a Medial Meniscectomy-Induced Animal Model Using Contrast-Enhanced X-ray Microtomography. Biomedicines 2020, 8, 56.

Journal reference: Biomedicines 2020, 8, 56
DOI: 10.3390/biomedicines8030056

Abstract

The aim of this study was to clarify degradation characteristics in each tissue of the knee complex of a medial meniscectomy (MMx)-induced knee osteoarthritis (KOA) animal model using classical methods and a new comprehensive evaluation method called contrast-enhanced X-ray micro-computed tomography (CEX-μCT), which was developed in the study. Surgical MMx was performed in the right knee joints of five male Wistar rats to induce KOA. At 4 wk post-surgery, the synovitis was evaluated using qPCR. Degradations of the articular cartilage of the tibial plateau were evaluated using classical methods and CEX-μCT. Evaluation of the synovitis demonstrated significantly increased expression levels of inflammation-associated marker genes in MMx-treated knees compared to that in sham-treated knees. Evaluation of the articular cartilage using classical methods showed that MMx fully induced degradation of the cartilage. Evaluation using CEX-μCT showed that local areas of the medial cartilage of the tibial plateau were significantly reduced in MMx-treated knees compared to that in sham-treated knees. On the other hand, total cartilage volumes were significantly increased in MMx-treated knees. Based on the findings of this study, the researchers in KOA research could be helped to select an optimal KOA model to discover new drugs.

Subject Areas

osteoarthritis; synovitis; articular cartilage; microfocus X-ray CT; 3D analysis

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