Working Paper Article Version 1 This version is not peer-reviewed

The Effects of 1-Methylcyclopropene (1-MCP) and Ethylene on Lignification of Postharvest Common Bean (Phaseolus vulgaris L)

Version 1 : Received: 12 January 2020 / Approved: 12 January 2020 / Online: 12 January 2020 (17:05:00 CET)

How to cite: Xie, G.; Feng, Y.; Chen, Y.; Zhang, M. The Effects of 1-Methylcyclopropene (1-MCP) and Ethylene on Lignification of Postharvest Common Bean (Phaseolus vulgaris L). Preprints 2020, 2020010131 Xie, G.; Feng, Y.; Chen, Y.; Zhang, M. The Effects of 1-Methylcyclopropene (1-MCP) and Ethylene on Lignification of Postharvest Common Bean (Phaseolus vulgaris L). Preprints 2020, 2020010131

Abstract

Postharvest 1-MCP treatment can inhibit lignification of fruit and vegetables. It has been suggested that the mode of action of 1-MCP is through inhibiting ethylene production, but the effect of 1-MCP and ethylene on lignification of common bean remain unknown. This work compared the effect of 0.5 μL L-1 1-MCP and 100 μL L-1 ethylene on lignification of common bean during storage. Postharvest 1-MCP significantly inhibited the increase of lignified cell group, sclerenchyma became thicker, vascular bundles thickened and lignified cells grew during storage, while ethylene is the opposite. 1-MCP inhibited the increase in respiration rate, sucrose phosphate synthase (SPS), sucrose synthase (SuSy), phenylalanine ammonialyase (PAL), cinnamyl-alcohol dehydrogenase (CAD), and peroxidase (POD) activities, whereas ethylene hastened all. Ethylene treatment can be stimulated and 1-MCP inhibited the decline of reducing sugar and cellulose content. Expression of genes, including PvACO1, PvAOG1, PvSuSy, PvPAL3, and Pv4CL1, PvCOMT1 together with lignin content were significantly increased in common bean during storage. 1-MCP treatment markedly inhibited expression of PvACO1, PvSuSy2, PvPAL3, Pv4CL1 and PvCOMT1 genes, while strengthened expression of PvETR1 and PvAOG1, while ethylene was opposite. This work provides evidence that ethylene plays a key role in regulating the lignin biosynthesis of common bean, and also provides strategies for the maintenance of fruit quality during storage.

Subject Areas

phaseolus vulgaris; ethylene; 1-MCP; lignification; storage

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