Working Paper Article Version 1 This version is not peer-reviewed

Evolution of the Complete Genome Sequence of Sacbrood Virus from Sichuan Province of China

Version 1 : Received: 3 January 2020 / Approved: 5 January 2020 / Online: 5 January 2020 (15:49:12 CET)

How to cite: OuYang, B.; Zhong, Y.; Lai, K.; Wang, J.; He, Y.; Xu, F.; Yang, J. Evolution of the Complete Genome Sequence of Sacbrood Virus from Sichuan Province of China. Preprints 2020, 2020010041 OuYang, B.; Zhong, Y.; Lai, K.; Wang, J.; He, Y.; Xu, F.; Yang, J. Evolution of the Complete Genome Sequence of Sacbrood Virus from Sichuan Province of China. Preprints 2020, 2020010041

Abstract

The Chinese honeybee (Apis cerana cerana) sacbrood virus (CSBV) causes death of larvae and colony collapse, and could damage the beekeeping industry in China. We sequenced complete genomes of CSBV strains derived from the Maerkang area, Wenjiang area, and Wanyuan area of Sichuan province of China. The genome length of CSBV strains from Sichuan was 8863bp, and it contained one complete Open Reading Frame of a gene with 8544 bp that encoded a protein with 2848 amino acids. The (G+C) % and (A+T) % composition ranged from 40.6 to 40.7 and 59.3 to 59.4, respectively. A phylogenetic tree was constructed using the three CSBV strains and previously reported SBV and CSBV sequences from other regions. We found that viral strains clustered based on their region of origin and host species. The genetic sequences of the CSBV strain from Maerkang were 98.7% and 99.6% similar to CSBV strains from Wanyuan and Wenjiang, respectively. In addition, CSBV from Maerkang had 88.4%-95.2% sequence similarity to previously published genomes of CSBV or SBV from other areas. The VP1 gene sequenced in our study had a 43 bp deletion compared to VP1 sequences of CSBV from other regions in Asia. We detected 10 antigenic determinants on the VP1 protein of CSBV form Aba. Our study provides new insight into the diversity of CSBV strains in China and may help with identifying methods to prevent infection of honeybee colonies.

Subject Areas

Chinese sacbrood virus; VP1; Apis cerana; Phylogenetic analysis

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