Preprint Technical Note Version 1 Preserved in Portico This version is not peer-reviewed

Microbiological Methods to Detect Intestinal Carriage of Highly-resistant Microorganisms (HRMO) in Humans and Livestock in the i-4-1-Health Dutch-Belgian Cross-border Project

Version 1 : Received: 13 December 2019 / Approved: 16 December 2019 / Online: 16 December 2019 (11:34:12 CET)

How to cite: Kluytmans-van den Bergh, M.; Lammens, C.; Perales Selva, N.; Verhulst, C.; Buiting, A.; Leroux-Roels, I.; Saegeman, V.; Savelkoul, P.; Schuermans, A.; Veenemans, J.; van der Vegt, D.; Willemsen, I.; Wintermans, B.; Goossens, H.; Kluytmans, J. Microbiological Methods to Detect Intestinal Carriage of Highly-resistant Microorganisms (HRMO) in Humans and Livestock in the i-4-1-Health Dutch-Belgian Cross-border Project . Preprints 2019, 2019120216. https://doi.org/10.20944/preprints201912.0216.v1 Kluytmans-van den Bergh, M.; Lammens, C.; Perales Selva, N.; Verhulst, C.; Buiting, A.; Leroux-Roels, I.; Saegeman, V.; Savelkoul, P.; Schuermans, A.; Veenemans, J.; van der Vegt, D.; Willemsen, I.; Wintermans, B.; Goossens, H.; Kluytmans, J. Microbiological Methods to Detect Intestinal Carriage of Highly-resistant Microorganisms (HRMO) in Humans and Livestock in the i-4-1-Health Dutch-Belgian Cross-border Project . Preprints 2019, 2019120216. https://doi.org/10.20944/preprints201912.0216.v1

Abstract

The i-4-1-Health project is a One Health project on the prevalence and spread of antimicrobial resistance in the human and veterinary domain in the Dutch-Belgian cross-border region. This paper describes the main components of the laboratory protocol that was developed to standardise the microbiological methods used for the detection of intestinal carriage of highly-resistant microorganisms.

Keywords

Antimicrobial resistance; intestinal carriage; detection; extended-spectrum beta-lactamase; carbapenem resistance; colistin resistance; ciprofloxacin resistance; vancomcin resistance; Enterobacterales; enterococci

Subject

Biology and Life Sciences, Immunology and Microbiology

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