Version 1
: Received: 11 December 2019 / Approved: 12 December 2019 / Online: 12 December 2019 (05:07:02 CET)
Version 2
: Received: 15 January 2020 / Approved: 16 January 2020 / Online: 16 January 2020 (05:13:40 CET)
Version 3
: Received: 14 April 2020 / Approved: 15 April 2020 / Online: 15 April 2020 (07:59:18 CEST)
How to cite:
Lainetti, P.D.F.; Brandi, A.; Filho, A.F.L.; Prado, M.C.M.; Kobayashi, P.E.; Laufer-Amorim, R.; Fonseca-Alves, C.E. Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines with Vasculogenic Mimicry Ability in vitro and in vivo. Preprints2019, 2019120162. https://doi.org/10.20944/preprints201912.0162.v1
Lainetti, P.D.F.; Brandi, A.; Filho, A.F.L.; Prado, M.C.M.; Kobayashi, P.E.; Laufer-Amorim, R.; Fonseca-Alves, C.E. Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines with Vasculogenic Mimicry Ability in vitro and in vivo. Preprints 2019, 2019120162. https://doi.org/10.20944/preprints201912.0162.v1
Lainetti, P.D.F.; Brandi, A.; Filho, A.F.L.; Prado, M.C.M.; Kobayashi, P.E.; Laufer-Amorim, R.; Fonseca-Alves, C.E. Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines with Vasculogenic Mimicry Ability in vitro and in vivo. Preprints2019, 2019120162. https://doi.org/10.20944/preprints201912.0162.v1
APA Style
Lainetti, P.D.F., Brandi, A., Filho, A.F.L., Prado, M.C.M., Kobayashi, P.E., Laufer-Amorim, R., & Fonseca-Alves, C.E. (2019). Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines with Vasculogenic Mimicry Ability <em>in vitro</em> and <em>in vivo</em>. Preprints. https://doi.org/10.20944/preprints201912.0162.v1
Chicago/Turabian Style
Lainetti, P.D.F., Renée Laufer-Amorim and Carlos Eduardo Fonseca-Alves. 2019 "Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines with Vasculogenic Mimicry Ability <em>in vitro</em> and <em>in vivo</em>" Preprints. https://doi.org/10.20944/preprints201912.0162.v1
Abstract
Mammary neoplasms affect a population of uncastrated and elderly female dogs and most of these neoplasms are malignant. In order to study this disease cell culture presents itself as a promising preclinical model, creating the opportunity to deposit cell lines at a cell bank, allowing a great repetition of the assays and making the validation of the results more reliable. Including, in vitro experiments for vasculogenic mimicry (VM) evaluation. VM is related to cancer cells capable of generate vascular-like structures without endothelial cells, mimicking the vasculogenic process. The aim of this study was to establish and characterize ten cell lines from canine mammary gland tumour according to immunophenotype and tumorigenicity, and with its ability to form vasculogenic mimicry-like structures in vitro. Fifteen samples from canine mammary gland carcinoma were collected and cultured in vitro and ten cell lines were established and characterized. Cells were evaluated for morphology, phenotype, vascular mimicry and tumorigenicity. All cell lines presented spindle shape morphology and expressed concomitant pan-cytokeratin and CK8/18. Four cell lines had vasculogenic mimicry ability and two cell showed in vivo tumorigenic potential. Cell characterization of those lines will help to create a database for more knowledge of mammary gland carcinomas in dogs, including studies of tumor behavior and new therapeutic targets.
Medicine and Pharmacology, Oncology and Oncogenics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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The commenter has declared there is no conflict of interests.
Comment:
Two things need to be done before this article is published:
-1) Provide the breed and age at sampling of the donor dogs.
-2) Change the name of the cell lines: names like CMnn (example CM1) or MMn (example MM3) are too short and collide with the names of other existing cell lines. You should prefix these names with the abbreviation of your insitution, examples: UNESP-CM1.
Commenter:
The commenter has declared there is no conflict of interests.
-1) Provide the breed and age at sampling of the donor dogs.
-2) Change the name of the cell lines: names like CMnn (example CM1) or MMn (example MM3) are too short and collide with the names of other existing cell lines. You should prefix these names with the abbreviation of your insitution, examples: UNESP-CM1.
Best regards and good luck with the publication