Sotnikov, D.V.; Barshevskaya, L.V.; Zherdev, A.V.; Eskendirova, S.Z.; Mukanov, K.K.; Mukantayev, K.K.; Ramanculov, Y.M.; Dzantiev, B.B. Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots. Appl. Sci.2020, 10, 738.
Sotnikov, D.V.; Barshevskaya, L.V.; Zherdev, A.V.; Eskendirova, S.Z.; Mukanov, K.K.; Mukantayev, K.K.; Ramanculov, Y.M.; Dzantiev, B.B. Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots. Appl. Sci. 2020, 10, 738.
Sotnikov, D.V.; Barshevskaya, L.V.; Zherdev, A.V.; Eskendirova, S.Z.; Mukanov, K.K.; Mukantayev, K.K.; Ramanculov, Y.M.; Dzantiev, B.B. Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots. Appl. Sci.2020, 10, 738.
Sotnikov, D.V.; Barshevskaya, L.V.; Zherdev, A.V.; Eskendirova, S.Z.; Mukanov, K.K.; Mukantayev, K.K.; Ramanculov, Y.M.; Dzantiev, B.B. Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots. Appl. Sci. 2020, 10, 738.
Abstract
In this article, we describe an immunochromatographic test system developed for rapid serodiagnostics of cattle brucellosis using two markers: gold nanoparticles (GNPs) and quantum dots (QDs). The test system was compared with immunochromatographic serodiagnostics systems that use only one marker. The approbation of the test system was conducted on samples of cattle sera with low, but diagnostically significant titers of specific antibodies. We show that when two conjugates are used, the intensity of the detectable signal increases by 2–3 times compared with the test system using the QD conjugate and by more than 9 times compared with the system using the GNP conjugate.
Biology and Life Sciences, Biology and Biotechnology
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
