Working Paper Article Version 1 This version is not peer-reviewed

Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots

Version 1 : Received: 27 November 2019 / Approved: 28 November 2019 / Online: 28 November 2019 (11:07:05 CET)

How to cite: Sotnikov, D.; Barshevskaya, L.; Zherdev, A.; Eskendirova, S.; Mukanov, K.; Mukantayev, K.; Ramankulov, Y.; Dzantiev, B. Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots. Preprints 2019, 2019110360 Sotnikov, D.; Barshevskaya, L.; Zherdev, A.; Eskendirova, S.; Mukanov, K.; Mukantayev, K.; Ramankulov, Y.; Dzantiev, B. Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots. Preprints 2019, 2019110360

Abstract

In this article, we describe an immunochromatographic test system developed for rapid serodiagnostics of cattle brucellosis using two markers: gold nanoparticles (GNPs) and quantum dots (QDs). The test system was compared with immunochromatographic serodiagnostics systems that use only one marker. The approbation of the test system was conducted on samples of cattle sera with low, but diagnostically significant titers of specific antibodies. We show that when two conjugates are used, the intensity of the detectable signal increases by 2–3 times compared with the test system using the QD conjugate and by more than 9 times compared with the system using the GNP conjugate.

Subject Areas

veterinary diagnostics; lateral flow tests; rapid tests; functionalized nanoparticles; signal enhancement; cow diseases

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