Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Up-Down and Left-Right by the Heart Transcriptome

Version 1 : Received: 19 November 2019 / Approved: 20 November 2019 / Online: 20 November 2019 (15:58:20 CET)

How to cite: Iacobas, S.; Amuzescu, B.; Iacobas, D. Up-Down and Left-Right by the Heart Transcriptome. Preprints 2019, 2019110243. https://doi.org/10.20944/preprints201911.0243.v1 Iacobas, S.; Amuzescu, B.; Iacobas, D. Up-Down and Left-Right by the Heart Transcriptome. Preprints 2019, 2019110243. https://doi.org/10.20944/preprints201911.0243.v1

Abstract

Myocardium transcriptomes of mouse left and right atria and ventricles were profiled separately to identify the differences that might be responsible for the distinct functional roles of the four heart chambers. In total, 16,886 distinct unigenes have been quantified in all 16 samples collected from four adult male mice from the same litter. 15.76% of the quantified genes on the left and 16.5% on the right exhibited differential expression between the corresponding atrium and ventricle of the same side, while 5.8% in atria and 1.2% in ventricles were differently expressed between the left and the right. Beyond the differentially expressed genes, the study revealed distinct expression control and coordination of ion channels and genes within the cardiac muscle contraction, oxidative phosphorylation, glycolysis/glucogenesis, calcium and adrenergic signaling pathways. Interestingly, while expression of Ank2 (encoding ankyrin-B) oscillates in phase with all its binding partners in the left ventricle, the percentage of synergistically expressed partners of Ank2 is 15% and 37% in the left and right atria and 74% in the right ventricle. The analysis revealed also the high interventricular synchrony of the expression of ion channels.

Keywords

adrenergic signaling; ankyrin-B; calcium channel; calcium signaling; glycolysis; oxidative phosphorylation; potassium channel; sodium channel

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

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