Preprint Communication Version 1 This version is not peer-reviewed

Development of Populus alba L. and Populus tremula L. Species Specific Molecular Markers Based on the 5S rDNA Non-transcribed Spacer Polymorphism

Version 1 : Received: 14 November 2019 / Approved: 15 November 2019 / Online: 15 November 2019 (16:50:27 CET)

A peer-reviewed article of this Preprint also exists.

Alexandrov, O.S.; Karlov, G.I. The Development of Populus alba L. and Populus tremula L. Species Specific Molecular Markers Based on 5S rDNA Non-Transcribed Spacer Polymorphism. Forests 2019, 10, 1092. Alexandrov, O.S.; Karlov, G.I. The Development of Populus alba L. and Populus tremula L. Species Specific Molecular Markers Based on 5S rDNA Non-Transcribed Spacer Polymorphism. Forests 2019, 10, 1092.

Journal reference: Forests 2019, 10, 1092
DOI: 10.3390/f10121092

Abstract

The Populus L. genus unites tree species, which are botanically grouped into several sections. The species successfully hybridize both in the same section and between some sections. The poplar hybridization widely occurs in nature and in variety breeding. Therefore, the development of poplar species specific molecular markers is very actual. The effective markers for trees of the Aigeiros Daby section have been recently developed using the polymorphism of the 5S rDNA non-transcribed spacers (NTSs). In this article, the 5S rDNA NTS based markers were designed for several species of the Leuce Daby section. The alb9 marker amplifies one fragment with DNA matrix of P. alba and P. × canescens (natural hybrid P. alba × P. tremula). The alb2 marker works the same way except the case with P. bolleana. In this case, the amplification of three fragments was observed. The tremu1 marker amplification is detected with DNA matrix of P. tremula and P. × canescens. Thus, the developed markers may be applied as useful tool for the P. alba, P. tremula, P. × canescens and P. bolleana identification in such areas of plant science as botany, dendrology, genetics of populations, variety breeding etc.

Subject Areas

poplars; molecular markers; non-transcribed spacers (ntss); 5s rdna; interspecies hybridization; species identification

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.