Version 1
: Received: 11 November 2019 / Approved: 12 November 2019 / Online: 12 November 2019 (16:06:06 CET)
How to cite:
Cacchio, P.; Del Gallo, M. A Novel Approach to Isolation and Screening of Calcifying Bacteria for Biotechnological Applications. Preprints2019, 2019110130. https://doi.org/10.20944/preprints201911.0130.v1
Cacchio, P.; Del Gallo, M. A Novel Approach to Isolation and Screening of Calcifying Bacteria for Biotechnological Applications. Preprints 2019, 2019110130. https://doi.org/10.20944/preprints201911.0130.v1
Cacchio, P.; Del Gallo, M. A Novel Approach to Isolation and Screening of Calcifying Bacteria for Biotechnological Applications. Preprints2019, 2019110130. https://doi.org/10.20944/preprints201911.0130.v1
APA Style
Cacchio, P., & Del Gallo, M. (2019). A Novel Approach to Isolation and Screening of Calcifying Bacteria for Biotechnological Applications. Preprints. https://doi.org/10.20944/preprints201911.0130.v1
Chicago/Turabian Style
Cacchio, P. and Maddalena Del Gallo. 2019 "A Novel Approach to Isolation and Screening of Calcifying Bacteria for Biotechnological Applications" Preprints. https://doi.org/10.20944/preprints201911.0130.v1
Abstract
Bacterial Calcium-carbonate Precipitation (BCP) has been studied for multiple applications such as remediation, consolidation and cementation. Isolation and screening of strong calcifying bacteria is the main task of BCP-technique. In this paper we studied CaCO3 precipitation by different bacteria isolated from a rhizospheric soil in both solid and liquid media. It has been found, through culture-depending studies, that bacteria belonging to Actinobacteria, Gammaproteobacteria and Alphaproteobacteria are the dominant bacteria involved in CaCO3 precipitation in this environment. Pure and mixed cultures of selected strains were applied for sand biocementation experiments. Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDS) analyses of the biotreated samples revealed the biological nature of the cementation and the effectiveness of the biodeposition treatment by mixed cultures. X-ray diffraction (XRD) analysis confirmed that all the calcifying strains selected for sand biocementation precipitated CaCO3, mostly in the form of calcite. In this study Biolog® Eco-plate is evaluated as a useful method for a more targeted choice of the sampling site with the purpose of obtaining interesting candidates for BCP applications. Furthermore, ImageJ software was investigated, for the first time to our knowledge, as a potential method to screen high CaCO3 producer strains.
Biology and Life Sciences, Ecology, Evolution, Behavior and Systematics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
