Article
Version 1
This version is not peer-reviewed
Protective Effects of Icariin on Nicotine-induced Reproductive Toxicity in Male Mouse
Version 1
: Received: 3 October 2019 / Approved: 8 October 2019 / Online: 8 October 2019 (10:30:43 CEST)
Version 2 : Received: 9 October 2019 / Approved: 14 October 2019 / Online: 14 October 2019 (09:58:40 CEST)
Version 2 : Received: 9 October 2019 / Approved: 14 October 2019 / Online: 14 October 2019 (09:58:40 CEST)
How to cite: Ni, G.; Zhang, X.; Afedo, S.Y.; Rui, R. Protective Effects of Icariin on Nicotine-induced Reproductive Toxicity in Male Mouse. Preprints 2019, 2019100082 Ni, G.; Zhang, X.; Afedo, S.Y.; Rui, R. Protective Effects of Icariin on Nicotine-induced Reproductive Toxicity in Male Mouse. Preprints 2019, 2019100082
Abstract
Nicotine is a pharmacologically active component of tobacco which adversely affects the male reproductive system and fertility and Icariin (ICA) is the main active ingredient of Epimedium herba which has been used to treat several male reproductive problems. This study was aimed at investigating the protective or ameliorative effect of ICA against reproductive toxicity induced by intraperitoneal injection of nicotine in mice. Forty male mice were randomly divided into 4 groups: control, nicotine (0.75 mg/kg intraperitoneally), icariin (ICA, 75 mg/kg), and icariin plus nicotine (ICA + nicotine) group. After 35 days of treatment, the mice were weighed, sacrificed, and their reproductive parameters were collected and examined for further studies. In the nicotine-treated group, epididymal sperm density and serum testosterone concentrations significantly decreased relative to the control group. Nicotine also caused oxidative damage as shown by significant reduction in the activities of antioxidant enzymes and an elevation in Malondialdehyde (MDA) levels. Icariin on the other hand, improved the reduction in sperm characteristics, hormone levels, and activities of antioxidant enzymes alterations observed in the nicotine treated mice. These findings indicate that the nicotine-induced reproductive toxicity and oxidative damages on male reproductive tissues can be effectively attenuated by icariin.
Keywords
icariin; nicotine; sperm density; testosterone; antioxidant enzyme; male mice
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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