Preprint Brief Report Version 1 This version is not peer-reviewed

Indexes of Angiogenic Activation in Myocardial Samples of Patients with Advanced Chronic Heart Failure

Version 1 : Received: 4 October 2019 / Approved: 8 October 2019 / Online: 8 October 2019 (05:59:11 CEST)

A peer-reviewed article of this Preprint also exists.

Komici, K.; Gnemmi, I.; Sangiorgi, C.; Ricciardolo, F.L.M.; Rinaldi, M.; Di Stefano, A.; Eleuteri, E. Indexes of Angiogenic Activation in Myocardial Samples of Patients with Advanced Chronic Heart Failure. Medicina 2019, 55, 766. Komici, K.; Gnemmi, I.; Sangiorgi, C.; Ricciardolo, F.L.M.; Rinaldi, M.; Di Stefano, A.; Eleuteri, E. Indexes of Angiogenic Activation in Myocardial Samples of Patients with Advanced Chronic Heart Failure. Medicina 2019, 55, 766.

Journal reference: Medicina 2019, 55, 766
DOI: 10.3390/medicina55120766

Abstract

Background and Objectives: Ischemic and idiopathic heart failure are two different etiologies, however reactive cardiac fibrosis together with impaired vasculogenesis has been described in both of them. Implication of main proangiogenic factors as: angiogenin, agiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2) has been described mainly in experimental models of heart failure. However, differences in molecular pathways between these cardiomyopathies are still under investigation. In this short communication we aimed to evaluate and compare the expression of pro-angiogenic molecules in the heart tissue of patients with advanced chronic heart failure (CHF) of ischemic and idiopathic etiology. Methods and Results: Heart tissue from left ventricular walls was obtained at transplantation from ischemic heart disease (IHD), idiopathic cardiomyopathy (ICM) patients. Tissue samples were examined using immunohistochemistry for angiogenic molecules. Immunopositivity (I-pos) for angiopoietin-1 was mainly observed in the cardiomyocytes, while I-pos for Ang-2 and Tie-2 receptor mainly in endothelial cells. Procollagen-I (PICP), angiogenin, Ang-1, Tie-2 receptor, were similarly expressed in IHD and ICM patients. In contrast, endothelial immunopositivity for Ang-2 was higher in IHD samples compared to ICM (p=0.03). Conclusions: Ang-2 expression is different in heart tissue of ICM and ICM patients and distribution of Ang-1 and angiogenin is higher in cardiomyocytes, whereas Ang-2 higher in endothelial cells, suggesting a different pattern of angiogenic stimulation, or at least of altered endothelial integrity. This data may serve for further studies investigating angiogenesis signaling pathways and in HF of different etiology.

Subject Areas

heart failure; angiogenesis; angiopoietin-1; angiopoietin-2; cardiac fibrosis

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