Version 1
: Received: 4 October 2019 / Approved: 7 October 2019 / Online: 7 October 2019 (11:16:43 CEST)
How to cite:
Islam, M.S.; Shimasaki, K. Factors Affecting Bulblet Growth of Lilium sp. – A Timeline for Bulblet to Bulb Production Via in Vitro vs Conventional Pathway. Preprints2019, 2019100063. https://doi.org/10.20944/preprints201910.0063.v1
Islam, M.S.; Shimasaki, K. Factors Affecting Bulblet Growth of Lilium sp. – A Timeline for Bulblet to Bulb Production Via in Vitro vs Conventional Pathway. Preprints 2019, 2019100063. https://doi.org/10.20944/preprints201910.0063.v1
Islam, M.S.; Shimasaki, K. Factors Affecting Bulblet Growth of Lilium sp. – A Timeline for Bulblet to Bulb Production Via in Vitro vs Conventional Pathway. Preprints2019, 2019100063. https://doi.org/10.20944/preprints201910.0063.v1
APA Style
Islam, M.S., & Shimasaki, K. (2019). Factors Affecting Bulblet Growth of Lilium sp. – A Timeline for Bulblet to Bulb Production Via in Vitro vs Conventional Pathway. Preprints. https://doi.org/10.20944/preprints201910.0063.v1
Chicago/Turabian Style
Islam, M.S. and Kazuhiko Shimasaki. 2019 "Factors Affecting Bulblet Growth of Lilium sp. – A Timeline for Bulblet to Bulb Production Via in Vitro vs Conventional Pathway" Preprints. https://doi.org/10.20944/preprints201910.0063.v1
Abstract
Lily–belong to the genus Lilium is one of the top cut flowers worldwide. Production and propagation of bulblets in vitro is an important approach for high-volume production, but not proved satisfactory. Hence, the aim of this study was to describe and compare the performances of morphological characteristics of the lily bulb in vivo produced by in vitro and conventional culture method and compare the production timelines in vitro vs conventional culture method. In results, it seems clear that in vitro re-culture of lily bulblet was able to be sustained and maintained its growth and so, ontogenic development after their performance in soil. Our results demonstrate that in the conventional pathway, the course of bulblet growth to bulb and ontogenic development took 3–4 growing seasons to reach the adult flowering phase. On the other hand, along with the re-culture in vitro, the course of bulb growth and ontogenic development took 1–2 growing seasons to reach the adult flowering phase because of the increasing initial bulb size and advancement of ontogenic development. Other than bulblet production through bulb scale explant, this study represents the first report on the method of in vitro bulb production of lily through re-culture by in vitro pathway with a comparison of the timelines and ontogenic development obtained from in vitro versus conventional pathway.
Keywords
lilium sp.; in vitro protocol; conventional pathway; bulb production; timeline
Subject
Biology and Life Sciences, Plant Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
