Version 1
: Received: 2 October 2019 / Approved: 3 October 2019 / Online: 3 October 2019 (14:01:36 CEST)
How to cite:
Yan-hua, Q.; Wen-xiu, L.; Ru, W.; Zaib_un, N.; Yang, Y.; Xiao-xia, J.; Li-jie, Y.; Chao, C. Identification and Expression Analysis of Strigolactone Biosynthetic and Signaling Genes in Response to Salt Stress in Soybean (Glycine max). Preprints2019, 2019100041
Yan-hua, Q.; Wen-xiu, L.; Ru, W.; Zaib_un, N.; Yang, Y.; Xiao-xia, J.; Li-jie, Y.; Chao, C. Identification and Expression Analysis of Strigolactone Biosynthetic and Signaling Genes in Response to Salt Stress in Soybean (Glycine max). Preprints 2019, 2019100041
Yan-hua, Q.; Wen-xiu, L.; Ru, W.; Zaib_un, N.; Yang, Y.; Xiao-xia, J.; Li-jie, Y.; Chao, C. Identification and Expression Analysis of Strigolactone Biosynthetic and Signaling Genes in Response to Salt Stress in Soybean (Glycine max). Preprints2019, 2019100041
APA Style
Yan-hua, Q., Wen-xiu, L., Ru, W., Zaib_un, N., Yang, Y., Xiao-xia, J., Li-jie, Y., & Chao, C. (2019). Identification and Expression Analysis of Strigolactone Biosynthetic and Signaling Genes in Response to Salt Stress in Soybean (<em>Glycine max</em>). Preprints. https://doi.org/
Chicago/Turabian Style
Yan-hua, Q., Yu Li-jie and Chen Chao. 2019 "Identification and Expression Analysis of Strigolactone Biosynthetic and Signaling Genes in Response to Salt Stress in Soybean (<em>Glycine max</em>)" Preprints. https://doi.org/
Abstract
Strigolactones (SLs) are a novel emerging plant hormones, which play important roles in regulating plant organ development and environmental stress tolerance. Even though the SL related genes have been identified and well characterized in some plants. The information of SL related genes in soybean is not fully established yet, especially in response to salt stress. In this study, we identified nine SL biosynthesis genes: two D27, two CCD7, two CCD8, and three MAX1, and seven SL signaling genes: two D14, two MAX2 and three D53 in soybean genome. We found that SL biosynthesis and signaling genes are conserved during evolution in different species. Syntenic analysis of these genes revealed their location on nine chromosomes as well as existence of ten pairs of duplication genes. Moreover, plant hormone and stress-responsive elements were identified in the promoter regions of SL biosynthesis and signaling genes. By using quantitative real-time PCR (qRT-PCR), we confirmed that SL genes have different tissue expression in roots, stems and leaves. Further, we also explored the expression profiles of SL biosynthesis and signaling genes under salt stress. These results suggested that SL signaling genes may play important regulatory roles in response to salt stress. In conclusion, we identified and provided valuable information on the soybean SL biosynthesis and signaling genes, and established a foundation for further functional analysis of soybean SL related genes in response to salt stress.
Keywords
soybean; strigolactones; biosynthesis and signaling genes; expression patterns; salt stress
Subject
Biology and Life Sciences, Plant Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
