Preprint Article Version 1 This version is not peer-reviewed

Molecular and biological characterization of a new strawberry Cytorhabdovirus

Version 1 : Received: 18 September 2019 / Approved: 19 September 2019 / Online: 19 September 2019 (11:17:42 CEST)

How to cite: Fránová, J.; Přibylová, J.; Koloniuk, I. Molecular and biological characterization of a new strawberry Cytorhabdovirus. Preprints 2019, 2019090220 (doi: 10.20944/preprints201909.0220.v1). Fránová, J.; Přibylová, J.; Koloniuk, I. Molecular and biological characterization of a new strawberry Cytorhabdovirus. Preprints 2019, 2019090220 (doi: 10.20944/preprints201909.0220.v1).

Abstract

Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus Fragaria thus far. Here, we describe a novel rhabdovirus, tentatively named strawberry virus 1 (StrV-1), that infects F. ananassa and F. vesca plants. Genomic sequences of three distinct StrV-1 genotypes co-infecting a single F. ananassa host were obtained using combined Illumina and Ion Proton high-throughput sequencing. StrV-1 was transmitted to herbaceous plants via Aphis fabae and A. ruborum, further mechanically transmitted to Nicotiana occidentalis 37B and sub-transferred to N. benthamiana, N. benthamiana DCL2/4i, N. occidentalis 37B and Physalis floridana plants. Irregular chlorotic sectors on leaf blades and the multiplication of calyx leaves seem to be the diagnostic symptoms for StrV-1 on indexed F. vesca clones. StrV-1 was detected in asymptomatic grafted plants and in 49 out of 159 field strawberry samples via RT-PCR followed by Sanger sequencing. The bacilliform shape of the virions, which have a cytoplasm-limited distribution, their size, and phylogenetic relationships support the assignment of StrV-1 to a distinct species of the genus Cytorhabdovirus. Acyrthosiphon malvae, A. fabae and A. ruborum were shown to transmit StrV-1 under experimental conditions.

Subject Areas

novel cytorhabdovirus; high-throughput sequencing; aphid transmission; mechanical inoculation; grafting-mediated virus transmission; fragaria spp.; chaetosiphon fragaefolii

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.