Preprint Article Version 2 This version is not peer-reviewed

Perspectives on the Use of Hyaluronidase from the Red King Crab Hepatopancreas in Treating Filler Complications: In Vitro Investigations

Version 1 : Received: 30 August 2019 / Approved: 1 September 2019 / Online: 1 September 2019 (02:31:34 CEST)
Version 2 : Received: 14 October 2019 / Approved: 14 October 2019 / Online: 14 October 2019 (15:42:23 CEST)

How to cite: Ponomareva, T.; Sliadovskii, D.; Timchenko, M.; Molchanov, M.; Timchenko, A.; Sogorin, E. Perspectives on the Use of Hyaluronidase from the Red King Crab Hepatopancreas in Treating Filler Complications: In Vitro Investigations. Preprints 2019, 2019090001 (doi: 10.20944/preprints201909.0001.v2). Ponomareva, T.; Sliadovskii, D.; Timchenko, M.; Molchanov, M.; Timchenko, A.; Sogorin, E. Perspectives on the Use of Hyaluronidase from the Red King Crab Hepatopancreas in Treating Filler Complications: In Vitro Investigations. Preprints 2019, 2019090001 (doi: 10.20944/preprints201909.0001.v2).

Abstract

This study focused on hydrolysis of cosmetic fillers hyaluronic acid (HA) and kinetics of the HA hydrolysis using the homogenate of the red king crab hepatopancreas. Turbidimetric analysis of the reaction mixture revealed a bell-shaped time dependence of aggregation formation. It was shown that the obtained homogenate has the similar activity to the commercially available hyaluronidase. The atomic force microscopy (AFM) examination found that the HA fillers were represented by spherical-like structures. These structures were destroyed under the action of the homogenate of the red king crab hepatopancreas. NMR of the reaction mixture showed that HA degradation lasts for some days, but a maximum rate of the reaction is detected in the first hours of incubation. The preparation with hyaluronidase activity obtained from the red king crab hepatopancreas could be used as potentially safe product for treating filler complications.

Subject Areas

treating filler complications; hyaluronidase; red king crab; hepatopancreas; hyaluronic acid (HA); hyaluronidase activity; turbidimetric method; atomic force microscopy (AFM); nuclear magnetic resonance (NMR); Revofil Ultra filler; Hyaluform deep filler; Lydase; Liporase

Comments (1)

Comment 1
Received: 14 October 2019
Commenter: Evgeny Sogorin
Commenter's Conflict of Interests: Author
Comment: To expand the range of used methods the analysis of the kinetics of hydrolysis and products of
reaction was performed by NMR method. The results are presented in the edited article.
The references have been added.
+ Respond to this comment

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 1
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.