Preprint Article Version 1 This version is not peer-reviewed

Standardisation of an Agroinfiltration Protocol for Eggplant Fruits and Proving its Usefulness by Over-expressing the SmHQT Gene

Version 1 : Received: 10 August 2019 / Approved: 11 August 2019 / Online: 11 August 2019 (10:57:58 CEST)

How to cite: Kaushik, P. Standardisation of an Agroinfiltration Protocol for Eggplant Fruits and Proving its Usefulness by Over-expressing the SmHQT Gene. Preprints 2019, 2019080129 (doi: 10.20944/preprints201908.0129.v1). Kaushik, P. Standardisation of an Agroinfiltration Protocol for Eggplant Fruits and Proving its Usefulness by Over-expressing the SmHQT Gene. Preprints 2019, 2019080129 (doi: 10.20944/preprints201908.0129.v1).

Abstract

Eggplant is a fruit vegetable of family Solanaceae, and eggplant fruits are of different shape and sizes that render them as an ideal system for metabolic engineering. Here, we have developed an agroinfiltration protocol for the transient expression of a gene in the eggplant fruit using GUS bearing; pCAMBIA1304 vector. Thereafter, to prove the effectiveness of the developed protocol, we have used the eggplant hydroxycinnamoyl CoA-quinate transferase (SmHQT), which is the central enzyme studied to increase the chlorogenic acid content, in a gene construct with the specific promoter in a plant transformation vector (pBIN19). Also, in our cassette, we also co-expressed the P19 protein of Tomato bushy stunt virus (native promoter) to overexpress the protein. Overall, using the protocol, the chlorogenic content was increased by more than two folds in the transgenic tissues.

Subject Areas

agroinfiltration; eggplant; fruits; agrobacterium; SmHQT

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