Preprint Article Version 1 This version is not peer-reviewed

Biofilm-Associated Agr and Sar Quorum Sensing Systems of Methicillin-Resistant Staphylococcus Aureus are Inhibited by Fruit Extracts of Illicium Verum

Version 1 : Received: 6 August 2019 / Approved: 7 August 2019 / Online: 7 August 2019 (10:29:04 CEST)

How to cite: Sankar Ganesh, P.; Krishnamurthy, V.; ISwamy, K.; Suvaithenamudhan, S.; Amuthan, M.; Vimali, I.; Veera Ravi, A.; Sivakumar, S.; Dinakar, C.; Nagarajan, S.; Shankar, E.M. Biofilm-Associated Agr and Sar Quorum Sensing Systems of Methicillin-Resistant Staphylococcus Aureus are Inhibited by Fruit Extracts of Illicium Verum. Preprints 2019, 2019080096 (doi: 10.20944/preprints201908.0096.v1). Sankar Ganesh, P.; Krishnamurthy, V.; ISwamy, K.; Suvaithenamudhan, S.; Amuthan, M.; Vimali, I.; Veera Ravi, A.; Sivakumar, S.; Dinakar, C.; Nagarajan, S.; Shankar, E.M. Biofilm-Associated Agr and Sar Quorum Sensing Systems of Methicillin-Resistant Staphylococcus Aureus are Inhibited by Fruit Extracts of Illicium Verum. Preprints 2019, 2019080096 (doi: 10.20944/preprints201908.0096.v1).

Abstract

Background: Staphylococcus aureus (S. aureus) is an opportunistic pathogen and a predominant cause of life-threatening nosocomial infections. Drug resistance in S. aureus is attributed to production of biofilm, which is controlled largely by bacterial quorum sensing (QS) systems. Methodology: In vitro analysis of biofilm inhibition assay was performed using crystal violet staining assay, swarming motility, light microscopy and growth curve analyses. Identification of the major constituents of I. verum fruit extract was performed by GC-MS. Ligand-protein interaction was analyzed by molecular docking investigations. Results: The methanol extract of I. verum inhibited the growth of MRSA at the concentration of 4.8 mg/ml. At the sub-inhibitory concentration (2.4mg/ml), the extract showed significant reduction in biofilmogenesis. Light microscopy analysis confirmed the antibiofilm activity as well as the efficacy in disturbing biofilm architecture. A reduced swarming motility was observed at the lowest concentration of 2.4mg/ml. GC-MS analysis revealed anethol (AL) as the major constituent. The molecular docking analysis attributes the antibiofilm activity to an active ligand AL, which strongly interacted with the active site residues of AgrA and SarA proteins of S. aureus. Conclusion: We report the activities of I. verum to be immensely interfering with QS system and biofilm formation in MRSA.

Subject Areas

AgrA protein; biofilm; MRSA; quorum sensing; SarA protein; swarming

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.