Version 1
: Received: 1 August 2019 / Approved: 5 August 2019 / Online: 5 August 2019 (04:13:54 CEST)
How to cite:
Stefansson, M.Ö.; Baldursson, S.; Magnússon, K.P.; Eyþórsdóttir, A.; Einarsson, H. Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters. Preprints2019, 2019080040
Stefansson, M.Ö.; Baldursson, S.; Magnússon, K.P.; Eyþórsdóttir, A.; Einarsson, H. Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters. Preprints 2019, 2019080040
Stefansson, M.Ö.; Baldursson, S.; Magnússon, K.P.; Eyþórsdóttir, A.; Einarsson, H. Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters. Preprints2019, 2019080040
APA Style
Stefansson, M.Ö., Baldursson, S., Magnússon, K.P., Eyþórsdóttir, A., & Einarsson, H. (2019). Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters. Preprints. https://doi.org/
Chicago/Turabian Style
Stefansson, M.Ö., Arnheiður Eyþórsdóttir and Hjörleifur Einarsson. 2019 "Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters" Preprints. https://doi.org/
Abstract
The following study reports on the first thraustochytrid isolates identified from Iceland. They were collected from three different locations off the northern coast of the country (Location A, Skagaströnd; Location B, Hveravík; and Location C, Eyjafjörður). Using 18S rDNA sequence analysis, isolates from Locations A and B were identified within the Thraustochytrium kinnei species while other isolates within the Sicyoidochytrium minutum species when compared to other known strains. Cells isolated from Locations A (2.10 ± 0.70 g/L) and B (1.54 ± 0.17 g/L) produced more biomass than the ones isolated from Location C (0.43 ± 0.02 g/L). This study offers the first-time examination of the utility of byproducts from fisheries as a nitrogen source in media formulation for thraustochytrids. Experiments showed that isolates produced more biomass (per unit of substrate) when cultured on nitrogen of marine (2.55 ± 0.74 g/L) as compared to of commercial origin (1.06 ± 0.57 g/L). Glycerol (2.43 ± 0.56 g/L) was a better carbon source than glucose (1.84 ± 0.57 g/L) in growth studies. Fatty acid (FA) profiles showed that the isolates from Location C (S. minutum) had low ratios of monounsaturated (4.21 ± 2.96%) and omega-6 (0.68 ± 0.59%) FAs. However, the isolates also had high ratios of docosahexaenoic acid (DHA; 35.65 ± 1.73%) and total omega-3 FAs (40.39 ± 2.39%), indicating that they could serve as a source of marine oils for human consumption and in aquaculture feeds. The T. kinnei isolates from Location A could be used in biodiesel production due to their high ratios of monounsaturated (18.38 ± 6.27%) long chain (57.43 ± 8.27%) FAs.
single cell oil; biomass; PUFA; docosahexaenoic acid (DHA); fish byproducts; biodiesel
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
