Preprint Article Version 1 This version is not peer-reviewed

Genomic Analysis of the Polyvalent Bacteriophage FP01

Version 1 : Received: 11 July 2019 / Approved: 12 July 2019 / Online: 12 July 2019 (13:07:09 CEST)

How to cite: Vasquez, I.; Retamales, J.; Parra, B.; Robeson, J.; Santander, J. Genomic Analysis of the Polyvalent Bacteriophage FP01. Preprints 2019, 2019070169 (doi: 10.20944/preprints201907.0169.v1). Vasquez, I.; Retamales, J.; Parra, B.; Robeson, J.; Santander, J. Genomic Analysis of the Polyvalent Bacteriophage FP01. Preprints 2019, 2019070169 (doi: 10.20944/preprints201907.0169.v1).

Abstract

Recently the polyvalent bacteriophage FP01, isolated from wastewater in Valparaiso, Chile, was described to have lytic activity across species against Escherichia coli and Salmonella enterica serovars. Due to it polyvalent nature the bacteriophage FP01 could have potential application in food and agri-industry. Also, fundamental aspects of polyvalent bacteriophage biology are not well known. In this study we sequenced and describe the complete genome of the polyvalent phage FP01 (MH745368) using the nanopore technology. The bacteriophage FP01 genome has a 44,900 bp, double-stranded DNA with an average G+C content of 49.41% and 90 coding sequences (CDSs). We found that the phage FP01 critically depends on host factors for replication and transcription. Also, it has a critical lysogenic repressor pseudogene. Phylogenetic analyses indicated that the phage FP01 is closely related to phages lambda and P22. These results suggest that the phage FP01 could be a lytic variant of a lysogenic phage or acquired genes from lysogenic phages during host infection.

Subject Areas

polyvalent bacteriophage FP01, Escherichia coli, Salmonella, genome

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