Preprint Review Version 2 This version is not peer-reviewed

Methods to Grow Better Diffractive Protein Crystals Acquired through Space Experiments

Version 1 : Received: 10 May 2019 / Approved: 13 May 2019 / Online: 13 May 2019 (01:47:52 CEST)
Version 2 : Received: 13 December 2019 / Approved: 15 December 2019 / Online: 15 December 2019 (13:43:29 CET)

A peer-reviewed article of this Preprint also exists.

Hashizume, Y.; Inaka, K.; Furubayashi, N.; Kamo, M.; Takahashi, S.; Tanaka, H. Methods for Obtaining Better Diffractive Protein Crystals: From Sample Evaluation to Space Crystallization. Crystals 2020, 10, 78. Hashizume, Y.; Inaka, K.; Furubayashi, N.; Kamo, M.; Takahashi, S.; Tanaka, H. Methods for Obtaining Better Diffractive Protein Crystals: From Sample Evaluation to Space Crystallization. Crystals 2020, 10, 78.

Journal reference: Crystals 2020, 10, 78
DOI: 10.3390/cryst10020078

Abstract

We summarize how to obtain protein crystals from which better diffraction images can be obtained. In particular, we describe in detail the quality evaluation of the protein sample, the crystallization methods and crystallization conditions, the flash-cooling protection of the crystal, and the crystallization under a microgravity environment.

Subject Areas

protein crystallization; protein sample qualification; JAXA PCG; microgravity

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