Preprint Review Version 2 This version is not peer-reviewed

Methods to Grow Better Diffractive Protein Crystals Acquired through Space Experiments

Version 1 : Received: 10 May 2019 / Approved: 13 May 2019 / Online: 13 May 2019 (01:47:52 CEST)
Version 2 : Received: 13 December 2019 / Approved: 15 December 2019 / Online: 15 December 2019 (13:43:29 CET)

How to cite: Hashizume, Y.; Inaka, K.; Furubayashi, N.; Kamo, M.; Takahashi, S.; Tanaka, H. Methods to Grow Better Diffractive Protein Crystals Acquired through Space Experiments. Preprints 2019, 2019050140 (doi: 10.20944/preprints201905.0140.v2). Hashizume, Y.; Inaka, K.; Furubayashi, N.; Kamo, M.; Takahashi, S.; Tanaka, H. Methods to Grow Better Diffractive Protein Crystals Acquired through Space Experiments. Preprints 2019, 2019050140 (doi: 10.20944/preprints201905.0140.v2).

Abstract

We summarize how to obtain protein crystals from which better diffraction images can be obtained. In particular, we describe in detail the quality evaluation of the protein sample, the crystallization methods and crystallization conditions, the flash-cooling protection of the crystal, and the crystallization under a microgravity environment.

Subject Areas

protein crystallization; protein sample qualification; JAXA PCG; microgravity

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