Working Paper Review Version 1 This version is not peer-reviewed

Quantitative Analysis of Nuclear Lamins Imaged by Super-Resolution Light Microscopy

Version 1 : Received: 21 March 2019 / Approved: 22 March 2019 / Online: 22 March 2019 (15:55:59 CET)

A peer-reviewed article of this Preprint also exists.

Kittisopikul, M.; Virtanen, L.; Taimen, P.; Goldman, R.D. Quantitative Analysis of Nuclear Lamins Imaged by Super-Resolution Light Microscopy. Cells 2019, 8, 361. Kittisopikul, M.; Virtanen, L.; Taimen, P.; Goldman, R.D. Quantitative Analysis of Nuclear Lamins Imaged by Super-Resolution Light Microscopy. Cells 2019, 8, 361.

Journal reference: Cells 2019, 8, 361
DOI: 10.3390/cells8040361

Abstract

The nuclear lamina consists of a dense fibrous meshwork made of nuclear lamins, Type V intermediate filaments, and is ~14 nm thick according to recent cryo-electron tomography studies. Recent advances in light microscopy have extended the resolution to a scale allowing for the fine structure of the lamina to be imaged in the context of the whole nucleus. We review quantitative approaches to analyze the imaging data of the nuclear lamina as acquired by structured illumination microscopy (SIM) and single molecule localization microscopy (SMLM), as well as the requisite cell preparation techniques. In particular, we discuss the application of steerable filters and graph based methods to segment the structure of the four mammalian lamin isoforms (A, C, B1, and B2) and extract quantitative information.

Subject Areas

lamins; structured illumination microscopy; single molecule localization microscopy; steerable filters; computational geometry; delaunay triangulation; voronoi tessellation

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