Preprint Article Version 1 This version is not peer-reviewed

Polysome-Associated lncRNAs during Cardiomyogenesis of hESCs

Version 1 : Received: 13 March 2019 / Approved: 15 March 2019 / Online: 15 March 2019 (02:11:52 CET)

How to cite: Pereira, I.T.; Spangenberg, L.; Cabrera, G.; Dallagiovanna, B. Polysome-Associated lncRNAs during Cardiomyogenesis of hESCs. Preprints 2019, 2019030157 (doi: 10.20944/preprints201903.0157.v1). Pereira, I.T.; Spangenberg, L.; Cabrera, G.; Dallagiovanna, B. Polysome-Associated lncRNAs during Cardiomyogenesis of hESCs. Preprints 2019, 2019030157 (doi: 10.20944/preprints201903.0157.v1).

Abstract

Long non-coding RNAs (lncRNAs) have been found to be involved in many biological processes, including the regulation of cell differentiation, but a complete characterization of lncRNA is still lacking. Additionally, there is evidence that lncRNAs interact with ribosomes, raising questions about their functions in cells. Here, we used a developmentally staged protocol to induce cardiogenic commitment of hESCs and then investigated the differential association of lncRNAs with polysomes. Our results identified lncRNAs in both the ribosome-free and polysome-bound fractions during cardiogenesis and showed a very well-defined temporal lncRNA association with polysomes. Clustering of lncRNAs was performed according to the gene expression patterns during the five timepoints analyzed. In addition, differential lncRNA recruitment to polysomes was observed when comparing the differentially expressed lncRNAs in the ribosome-free and polysome-bound fractions or when calculating the polysome-bound vs ribosome-free ratio. The association of lncRNAs with polysomes could represent an additional cytoplasmic role of lncRNAs, e.g., in translational regulation of mRNA expression.

Subject Areas

long non-coding RNA; hESC; cardiomyocyte; RNA-seq

Comments (1)

Comment 1
Received: 27 March 2019
Commenter: Michael Alexanian
The commenter has declared there is no conflict of interests.
Comment: Very nice study that highlights the possibility of additional cytoplasmic roles of lncRNAs associated to polysomes. In the introduction the authors mentioned linc1405 as an example of lncRNA with an important role in cardiogenesis. The role of this lncRNA (also known as Meteor) in cardiogenesis was firstly described in Alexanian et al. (Nature Communications, 2017 - https://www.nature.com/articles/s41467-017-01804-w). A Meteor human ortholog that is regulated during human iPSC differentiation into cardiomyocytes was described in the same study. It would be interesting to know if Meteor is part of the lncRNA the authors identified in their assay.
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