Preprint Article Version 1 This version is not peer-reviewed

A Diet Based on Cured Acorn Ham with Oleic Acid Content Promotes Anti-Inflammatory Gut Microbiota Shifts and Prevents Ulcerative Colitis in an Animal Model

Version 1 : Received: 27 February 2019 / Approved: 1 March 2019 / Online: 1 March 2019 (08:42:26 CET)

How to cite: Fernández, J.; García de la Fuente, V.; Fernández García, M.T.; Gómez Sánchez, J.; Redondo, B.I.; Villar, C.J.; Lombó, F. A Diet Based on Cured Acorn Ham with Oleic Acid Content Promotes Anti-Inflammatory Gut Microbiota Shifts and Prevents Ulcerative Colitis in an Animal Model. Preprints 2019, 2019030005 (doi: 10.20944/preprints201903.0005.v1). Fernández, J.; García de la Fuente, V.; Fernández García, M.T.; Gómez Sánchez, J.; Redondo, B.I.; Villar, C.J.; Lombó, F. A Diet Based on Cured Acorn Ham with Oleic Acid Content Promotes Anti-Inflammatory Gut Microbiota Shifts and Prevents Ulcerative Colitis in an Animal Model. Preprints 2019, 2019030005 (doi: 10.20944/preprints201903.0005.v1).

Abstract

Background: Diets based on meat products are not recommended in the case of ulcerative colitis (UC). However, some foods, as those containing high oleic acid and a low omega-6/omega-3 ratio show anti-inflammatory properties. The objective here is to test if some traditional cured meat products, as acorn-fed ham (high levels of oleic acid), may be useful for controlling inflammatory diseases as UC in animal models. Methods: 3 rat cohorts have been used: vegetable rat feed, control ham and acorn-fed ham (a traditional ham where high oleic acid concentration from acorns is storage in the muscle fat). UC was induced with DSS in drinking water ad libitum for one week. Short-chain fatty acids (SCFAs) and 16S rRNA from bacterial populations were analyzed in cecum samples. Colon samples were analyzed for histological parameters (inflammatory cell density, mucosa damages, myeloperoxidase). Results: In the acorn-fed ham cohort, a protective effect was observed with respect to UC disease activity index, inflammatory cells density, colon mucosa alterations, myeloperoxidase levels, blood total antioxidant capacity and lower levels of pro-inflammatory cytokines, in comparison with feed cohort. Both ham diets caused a reduction in Firmicutes and an increase in Actinobacteria, Bacteroidetes and Proteobacteria in comparison with rat feed diet. Also, acorn-fed ham diet induced changes in gut microbiota composition, with pronounced enrichments in anti-inflammatory bacterial genera such as Alistipes, Bacteroides, Blautia, Butyricimonas and Parabacteroides. Conclusions: In the acorn-fed ham cohort, as a result of the dietary intake of oleic acid and low intake of omega-6 fatty acids, a strong preventive effect against UC symptoms was observed, indicating a valuable effect of this traditional Mediterranean cured meat product.

Subject Areas

oleic acid; acorn feed ham; gut microbiota; ulcerative colitis

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